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About
UniTrap UNI15309
Trapped Gene
Hnrph1 (
ENSMUSG00000007850
)
Vector Insertion
Chr 11: 50197423 - 50198145
Public Clones
PST283-1 (escells)
PST11033-NR (escells)
Private Clones
not available
Severity of mutation
(?)
Insertion after 90% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000103884 (Chr11:50197333..50197422 +)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
No primer for this exon
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000103884 (Chr11:50197333..50197422 +)
Downstram Exon
ENSMUSE00000471098 (Chr11:50198146..50198238 +)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
No primer for this exon
No primer for this exon
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000679162
Chr11:50190492..50190649
No primer for this exon
upstream
ENSMUSE00000679161
Chr11:50190816..50190942
No primer for this exon
upstream
ENSMUSE00000103891
Chr11:50191286..50191304
No primer for this exon
upstream
ENSMUSE00000103889
Chr11:50191793..50191929
No primer for this exon
upstream
ENSMUSE00000721161
Chr11:50191793..50191929
No primer for this exon
upstream
ENSMUSE00000103875
Chr11:50192970..50193125
No primer for this exon
upstream
ENSMUSE00000103873
Chr11:50193323..50193466
No primer for this exon
upstream
ENSMUSE00000479443
Chr11:50194961..50195099
No primer for this exon
upstream
ENSMUSE00000103885
Chr11:50196039..50196217
No primer for this exon
upstream
ENSMUSE00000103883
Chr11:50196302..50196373
No primer for this exon
upstream
ENSMUSE00000103887
Chr11:50196430..50196563
No primer for this exon
upstream
ENSMUSE00000513096
Chr11:50196681..50196816
No primer for this exon
upstream
ENSMUSE00000496715
Chr11:50197182..50197241
No primer for this exon
upstream
ENSMUSE00000103884
Chr11:50197333..50197422
No primer for this exon
*** Putative Vector Insertion (Chr 11: 50197423 - 50198145) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000471098
Chr11:50198146..50198238
No primer for this exon
downstream
ENSMUSE00000103893
Chr11:50198643..50198692
No primer for this exon
downstream
ENSMUSE00000580013
Chr11:50199279..50200029
No primer for this exon
downstream
ENSMUSE00000679160
Chr11:50199279..50200030
No primer for this exon
downstream
ENSMUSE00000679163
Chr11:50199279..50200029
No primer for this exon
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
AAGTAATCGCCTTGCAGCAC
Chr11:50197471..50197491
60.42
50
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
AGCGGTGGTGCTTATGGTAG
Chr11:50197378..50197398
60.15
55
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000007850
