Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI15420
Trapped Gene
Arhgap17 (ENSMUSG00000030766)
Vector Insertion
Chr 7: 130350804 - 130423631
Public Clones 3SD051E10 (ggtc) 3SD121B08 (ggtc) PST7319-NL (escells) IST14193G4 (tigm)
IST14699D2 (tigm) IST10300G6 (tigm) IST10708F3 (tigm) IST12936F8 (tigm)
IST13863C3 (tigm) IST10744F3 (tigm) IST14927A4 (tigm) IST13511G3 (tigm)
IST12709A2 (tigm) IST10580A4 (tigm) IST10908D2 (tigm) IST12174C7 (tigm)
IST13068F7 (tigm) IST12951F3 (tigm) IST10850E11 (tigm) IST10251D4 (tigm)
IST14914F12 (tigm) IST12608F4 (tigm) IST12422B12 (tigm) IST10785G8 (tigm)
IST13376D7 (tigm) IST11916B1 (tigm) IST11987C3 (tigm) IST14764F12 (tigm)
IST12801E5 (tigm) IST10490F1 (tigm) IST14231C1 (tigm) IST10744F3 (tigm)
IST12709A2 (tigm) IST10852D9 (tigm) IST10490F1 (tigm) IST14495H12 (tigm)
IST14963B12 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 100% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000632570 (Chr7:130422785..130423630 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CATTCAGACTTGGCAAGCAA Chr7:130423561..130423580 59.99 45 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000632570 (Chr7:130422785..130423630 -)
Downstram Exon
ENSMUSE00000669940 (Chr7:130350805..130350818 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CATTCAGACTTGGCAAGCAA Chr7:130423561..130423580 59.99 45 No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000669939 Chr7:130513268..130513392 GTAGGTAGGACCCGCCAGAC Chr7:130513373..130513392 60.9 65
upstream ENSMUSE00000669962 Chr7:130513268..130513405 GTAGGTAGGACCCGCCAGAC Chr7:130513373..130513392 60.9 65
upstream ENSMUSE00000632588 Chr7:130474740..130474779 AGCTGAGAAGACCGAAGTCC Chr7:130474760..130474779 58.62 55
upstream ENSMUSE00000632587 Chr7:130470753..130470857 CTGGACACTGTGCGTTCAAT Chr7:130470826..130470845 59.75 50
upstream ENSMUSE00000632586 Chr7:130467127..130467200 AGAACATGCAGGAGGCTTCA Chr7:130467153..130467172 60.94 50
upstream ENSMUSE00000632585 Chr7:130465393..130465504 TGGACCCTCTCTACGGCATA Chr7:130465399..130465418 60.62 55
upstream ENSMUSE00000632584 Chr7:130465102..130465178 GGTGTTGGACTGGGATTCAG Chr7:130465112..130465131 60.36 55
upstream ENSMUSE00000632583 Chr7:130461901..130462012 AGGGGCTTCCATCCAAAATA Chr7:130461955..130461974 60.63 45
upstream ENSMUSE00000632582 Chr7:130458158..130458226 GCCAAAGAAGGGGAGTATGG Chr7:130458174..130458193 60.82 55
upstream ENSMUSE00000632580 Chr7:130457928..130458009 GAAGCCCAAGCAGATTACCA Chr7:130457984..130458003 60.21 50
upstream ENSMUSE00000632579 Chr7:130451800..130451927 GGAGACTGGCATGAAAGAGG Chr7:130451802..130451821 59.8 55
upstream ENSMUSE00000632578 Chr7:130450482..130450593 AAAGCTGCTCTCGACTGCTC Chr7:130450529..130450548 60.04 55
upstream ENSMUSE00000632577 Chr7:130449889..130449970 AGTCCTATCTGCGGGAGTTG Chr7:130449942..130449961 59.31 55
upstream ENSMUSE00000632576 Chr7:130447210..130447290 AGAAGTTGCCGCCACAAAAT Chr7:130447224..130447243 61.91 45
upstream ENSMUSE00000632575 Chr7:130444003..130444116 GCAACATAGCCATTGTCCTG Chr7:130444035..130444054 59.15 50
upstream ENSMUSE00000632574 Chr7:130441009..130441100 AGTGATTGAGCCCATCATCC Chr7:130441036..130441055 59.89 50
upstream ENSMUSE00000632573 Chr7:130439924..130440080 TGACTCTGACTCGGGGACTC Chr7:130439985..130440004 60.4 60
upstream ENSMUSE00000202967 Chr7:130437986..130438219 CTACGGGCATATTGGAGCAG Chr7:130438009..130438028 60.62 55
upstream ENSMUSE00000632572 Chr7:130435550..130435719 GAACAGCAACCAGATGACCA Chr7:130435649..130435668 59.68 50
upstream ENSMUSE00000632592 Chr7:130430130..130430527 ATCCATCGCAAAGTGAGACC Chr7:130430163..130430182 60.08 50
upstream ENSMUSE00000632591 Chr7:130430096..130430128 No primer for this exon
upstream ENSMUSE00000632590 Chr7:130430013..130430093 ATGGTGGCCTTACATCCTCA Chr7:130430040..130430059 60.34 50
upstream ENSMUSE00000632571 Chr7:130430012..130430527 ATCCATCGCAAAGTGAGACC Chr7:130430163..130430182 60.08 50
upstream ENSMUSE00000669935 Chr7:130429816..130429820 No primer for this exon
upstream ENSMUSE00000669938 Chr7:130428934..130428981 CTCACAGGTGGGGAAGGTTT Chr7:130428945..130428964 61.31 55
upstream ENSMUSE00000669936 Chr7:130422789..130423630 CATTCAGACTTGGCAAGCAA Chr7:130423561..130423580 59.99 45
upstream ENSMUSE00000632570 Chr7:130422785..130423630 CATTCAGACTTGGCAAGCAA Chr7:130423561..130423580 59.99 45
upstream ENSMUSE00000669940 Chr7:130350805..130350818 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ACCTGCTTTTGATCCCACAC Chr7:130369610..130369630 59.97 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ACCTGCTTTTGATCCCACAC Chr7:130369610..130369630 59.97 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000030766