Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI15430
Trapped Gene
Btf3l4 (ENSMUSG00000028568)
Vector Insertion
Chr 4: 108489054 - 108490850
Public Clones PST6781-NR (escells)
Private Clones not available
Severity of mutation (?) Insertion after 91% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000662463 (Chr4:108490790..108490849 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TAGTAAAGCGCCCAAACCAG Chr4:108490823..108490842 60.26 50 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000662463 (Chr4:108490790..108490849 -)
Downstram Exon
ENSMUSE00000662462 (Chr4:108489055..108489491 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TAGTAAAGCGCCCAAACCAG Chr4:108490823..108490842 60.26 50 CAAATGACCGAGAACTGCTG Chr4:108489264..108489283 59.44 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000662460 Chr4:108506113..108506159 No primer for this exon
upstream ENSMUSE00000662466 Chr4:108506113..108506219 GAGGCAGCCATCTTGCTCTT Chr4:108506150..108506169 61.97 55
upstream ENSMUSE00000662461 Chr4:108505748..108506169 CAGTGTCAGACGCTTTGAGC Chr4:108505789..108505808 59.78 55
upstream ENSMUSE00000662459 Chr4:108505018..108505056 No primer for this exon
upstream ENSMUSE00000662457 Chr4:108504311..108504536 TGCTGCACAGCTAAGATTGG Chr4:108504469..108504488 60.16 50
upstream ENSMUSE00000662465 Chr4:108503123..108503188 TCAAGCTCAGGTCCGGATAG Chr4:108503131..108503150 60.35 55
upstream ENSMUSE00000716151 Chr4:108503123..108503188 TCAAGCTCAGGTCCGGATAG Chr4:108503131..108503150 60.35 55
upstream ENSMUSE00000525636 Chr4:108498705..108498818 ACAGCTCGCAGGAAGAAGAA Chr4:108498796..108498815 60.28 50
upstream ENSMUSE00000662464 Chr4:108491741..108491942 CTCTCCGCTAACACCTTTGC Chr4:108491860..108491879 60.01 55
upstream ENSMUSE00000662463 Chr4:108490790..108490849 TAGTAAAGCGCCCAAACCAG Chr4:108490823..108490842 60.26 50
upstream ENSMUSE00000662456 Chr4:108489437..108489491 TGAAGCATCGAAAAATGAAGC Chr4:108489452..108489472 60.34 38.1
upstream ENSMUSE00000662458 Chr4:108489396..108489491 GGAAGCTGGCATGGACTAGA Chr4:108489410..108489429 60.36 55
upstream ENSMUSE00000601484 Chr4:108489323..108489491 TGAAGCATCGAAAAATGAAGC Chr4:108489452..108489472 60.34 38.1
upstream ENSMUSE00000662462 Chr4:108489055..108489491 GGGTGGAACTTTTGGTGTGT Chr4:108489159..108489178 59.72 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ATAGTAAAGCGCCCAAACCA Chr4:108490822..108490842 59.61 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ATAGTAAAGCGCCCAAACCA Chr4:108490822..108490842 59.61 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000028568