Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI15763
Trapped Gene
Prkcbp1 (ENSMUSG00000039671)
Vector Insertion
Chr 2: 165701204 - 165701276
Public Clones CMHD-GT_379G11-3 (cmhd) CMHD-GT_495D3-3 (cmhd) CMHD-GT_364B7-3 (cmhd) CMHD-GT_429G5-3 (cmhd)
CMHD-GT_540B6-3 (cmhd) CMHD-GT_364D12-3 (cmhd) CMHD-GT_379G3-3 (cmhd) CMHD-GT_357D5-3 (cmhd)
CMHD-GT_475E4-3 (cmhd) CMHD-GT_348C8-3 (cmhd) CMHD-GT_522D7-3 (cmhd)
Private Clones OST500678 (lexicon) OST470067 (lexicon) OST467862 (lexicon) OST467846 (lexicon)
OST465387 (lexicon) OST463001 (lexicon) OST461761 (lexicon) OST459576 (lexicon)
OST456760 (lexicon) OST455617 (lexicon) OST455095 (lexicon) OST450568 (lexicon)
OST449489 (lexicon) OST444544 (lexicon) OST443820 (lexicon) OST437720 (lexicon)
OST432789 (lexicon) OST430654 (lexicon) OST430618 (lexicon) OST427637 (lexicon)
OST426206 (lexicon) OST425335 (lexicon) OST425098 (lexicon) OST417460 (lexicon)
OST416847 (lexicon) OST415000 (lexicon) OST413681 (lexicon) OST412308 (lexicon)
OST406524 (lexicon) OST395011 (lexicon) OST393637 (lexicon) OST393238 (lexicon)
OST392150 (lexicon) OST390709 (lexicon) OST386399 (lexicon) OST384602 (lexicon)
OST383414 (lexicon) OST382329 (lexicon) OST378921 (lexicon) OST378251 (lexicon)
OST377299 (lexicon) OST375542 (lexicon) OST375371 (lexicon) OST368053 (lexicon)
OST367851 (lexicon) OST367513 (lexicon) OST366066 (lexicon) OST363767 (lexicon)
OST363653 (lexicon) OST358551 (lexicon) OST358383 (lexicon) OST354981 (lexicon)
OST347628 (lexicon) OST345463 (lexicon) OST344764 (lexicon) OST340422 (lexicon)
OST335893 (lexicon) OST335175 (lexicon) OST333793 (lexicon) OST329692 (lexicon)
OST328637 (lexicon) OST323615 (lexicon) OST323611 (lexicon) OST322706 (lexicon)
OST321851 (lexicon) OST319763 (lexicon) OST318532 (lexicon) OST312357 (lexicon)
OST311437 (lexicon) OST311411 (lexicon) OST310387 (lexicon) OST307941 (lexicon)
OST299430 (lexicon) OST292086 (lexicon) OST288210 (lexicon) OST284033 (lexicon)
OST282775 (lexicon) OST279655 (lexicon) OST279492 (lexicon) OST278486 (lexicon)
OST276898 (lexicon) OST275812 (lexicon) OST266782 (lexicon) OST263544 (lexicon)
OST263490 (lexicon) OST262071 (lexicon) OST259742 (lexicon) OST259601 (lexicon)
OST259247 (lexicon) OST257297 (lexicon) OST253321 (lexicon) OST253222 (lexicon)
OST246512 (lexicon) OST246145 (lexicon) OST236089 (lexicon) OST234819 (lexicon)
OST233848 (lexicon) OST233347 (lexicon) OST231594 (lexicon) OST218790 (lexicon)
OST216804 (lexicon) OST205970 (lexicon) OST205565 (lexicon) OST204976 (lexicon)
OST200444 (lexicon) OST200182 (lexicon) OST195173 (lexicon) OST192768 (lexicon)
OST190508 (lexicon) OST190091 (lexicon) OST189518 (lexicon) OST173402 (lexicon)
OST129489 (lexicon) OST124314 (lexicon) OST123677 (lexicon) OST118658 (lexicon)
OST115050 (lexicon) OST111137 (lexicon) OST107401 (lexicon) OST97779 (lexicon)
OST97775 (lexicon) OST97498 (lexicon) OST92643 (lexicon) OST91366 (lexicon)
OST81627 (lexicon) OST80599 (lexicon) OST80006 (lexicon) OST68654 (lexicon)
OST68165 (lexicon) OST67040 (lexicon) OST66993 (lexicon) OST66672 (lexicon)
OST65173 (lexicon) OST64978 (lexicon) OST64934 (lexicon) OST63655 (lexicon)
OST60794 (lexicon) OST60350 (lexicon) OST59935 (lexicon) OST59863 (lexicon)
OST57524 (lexicon) OST57038 (lexicon) OST56277 (lexicon) OST54562 (lexicon)
OST53937 (lexicon) OST53167 (lexicon) OST52893 (lexicon) OST52889 (lexicon)
OST50500 (lexicon) OST47208 (lexicon) OST47149 (lexicon) OST44128 (lexicon)
OST44084 (lexicon) OST43554 (lexicon) OST43015 (lexicon) OST42482 (lexicon)
OST41924 (lexicon) OST41166 (lexicon) OST39891 (lexicon) OST39690 (lexicon)
OST39605 (lexicon) OST39486 (lexicon) OST39397 (lexicon) OST39392 (lexicon)
OST39370 (lexicon) OST37355 (lexicon) OST36342 (lexicon) OST36009 (lexicon)
OST34734 (lexicon) OST34613 (lexicon) OST34377 (lexicon) OST34094 (lexicon)
OST33735 (lexicon) OST31008 (lexicon) OST30467 (lexicon) OST30323 (lexicon)
OST29625 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000717447 (Chr2:165701205..165701275 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TAAAAACCGAGCAGGAGGTG Chr2:165701239..165701258 60.24 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000717447 (Chr2:165701205..165701275 -)
Downstram Exon
ENSMUSE00000409419 (Chr2:165701205..165701275 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TAAAAACCGAGCAGGAGGTG Chr2:165701239..165701258 60.24 50 CACCTCCTGCTCGGTTTTTA Chr2:165701217..165701236 60.24 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000679658 Chr2:165724416..165724462 No primer for this exon
upstream ENSMUSE00000679660 Chr2:165722353..165722450 AGAGAATGCTGGGTTCTGGA Chr2:165722392..165722411 59.8 50
upstream ENSMUSE00000592815 Chr2:165710108..165710145 No primer for this exon
upstream ENSMUSE00000679676 Chr2:165710108..165710374 CCCGAGCCTCACAAAGATAA Chr2:165710304..165710323 60.21 50
upstream ENSMUSE00000679684 Chr2:165710108..165710272 CAATCGGGGATGAGCTTTTA Chr2:165710223..165710242 60.03 45
upstream ENSMUSE00000679637 Chr2:165709679..165709710 CTGGGGGAAGAAAATGGAAG Chr2:165709679..165709698 60.79 50
upstream ENSMUSE00000679683 Chr2:165708828..165708898 No primer for this exon
upstream ENSMUSE00000679694 Chr2:165707649..165707778 CACGCATTAAAATGGCTCCT Chr2:165707754..165707773 60.1 45
upstream ENSMUSE00000679682 Chr2:165701785..165703937 TTCCATGTTGGTGACATGCT Chr2:165702709..165702728 59.97 45
upstream ENSMUSE00000409419 Chr2:165701205..165701275 TAAAAACCGAGCAGGAGGTG Chr2:165701239..165701258 60.24 50
upstream ENSMUSE00000550359 Chr2:165701205..165701275 TAAAAACCGAGCAGGAGGTG Chr2:165701239..165701258 60.24 50
upstream ENSMUSE00000717447 Chr2:165701205..165701275 TAAAAACCGAGCAGGAGGTG Chr2:165701239..165701258 60.24 50

*** Putative Vector Insertion (Chr 2: 165701204 - 165701276) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000679677 Chr2:165678368..165678383 No primer for this exon
downstream ENSMUSE00000679681 Chr2:165678368..165678379 No primer for this exon
downstream ENSMUSE00000679636 Chr2:165677533..165677648 TCTTTTTAATGGGGCTCGTG Chr2:165677519..165677538 60.07 45
downstream ENSMUSE00000592814 Chr2:165677500..165677648 TCTTTTTAATGGGGCTCGTG Chr2:165677519..165677538 60.07 45
downstream ENSMUSE00000550346 Chr2:165671521..165671739 GTCCTGCGGTACAACATCAA Chr2:165671640..165671659 59.57 50
downstream ENSMUSE00000679691 Chr2:165671521..165671664 CTCTGGCTCCGATGTCAGTC Chr2:165671526..165671545 60.98 60
downstream ENSMUSE00000639167 Chr2:165668264..165668377 CGTCTCGATGCATTCTGCTA Chr2:165668326..165668345 60.12 50
downstream ENSMUSE00000710025 Chr2:165665498..165665590 AGTCAGGGTGTTGCTCCAAT Chr2:165665523..165665542 59.58 50
downstream ENSMUSE00000717483 Chr2:165665498..165665590 AGTCAGGGTGTTGCTCCAAT Chr2:165665523..165665542 59.58 50
downstream ENSMUSE00000639165 Chr2:165664312..165664399 CTTCTGTGCAGCCGTACATC Chr2:165664344..165664363 59.47 55
downstream ENSMUSE00000679656 Chr2:165664312..165664399 CTTCTGTGCAGCCGTACATC Chr2:165664344..165664363 59.47 55
downstream ENSMUSE00000639163 Chr2:165662781..165662836 TGCTATTTGCGTCAACTTGTG Chr2:165662789..165662809 59.93 42.86
downstream ENSMUSE00000679655 Chr2:165662781..165662836 TGCTATTTGCGTCAACTTGTG Chr2:165662789..165662809 59.93 42.86
downstream ENSMUSE00000639162 Chr2:165660390..165660467 TTTTGGCAAGCTGCAAGATA Chr2:165660396..165660415 59.58 40
downstream ENSMUSE00000679654 Chr2:165660390..165660467 TTTTGGCAAGCTGCAAGATA Chr2:165660396..165660415 59.58 40
downstream ENSMUSE00000639161 Chr2:165658809..165658924 CCTTTCAGTTTTGCCCAGAC Chr2:165658865..165658884 59.71 50
downstream ENSMUSE00000679653 Chr2:165658809..165658924 CCTTTCAGTTTTGCCCAGAC Chr2:165658865..165658884 59.71 50
downstream ENSMUSE00000520710 Chr2:165653708..165654189 TACTGGTTGTTGGGCGTGTA Chr2:165653982..165654001 60.03 50
downstream ENSMUSE00000639160 Chr2:165653708..165654189 TACTGGTTGTTGGGCGTGTA Chr2:165653982..165654001 60.03 50
downstream ENSMUSE00000679659 Chr2:165652682..165654189 AGTTACGGTCGCCATGGTAG Chr2:165652972..165652991 60.01 55
downstream ENSMUSE00000639159 Chr2:165646042..165646188 TGGAAGTCTTGTCGGGTTTT Chr2:165646056..165646075 59.57 45
downstream ENSMUSE00000679651 Chr2:165646042..165646188 TGGAAGTCTTGTCGGGTTTT Chr2:165646056..165646075 59.57 45
downstream ENSMUSE00000639158 Chr2:165640817..165640969 GACTCGCTCAGCTCCTTCAG Chr2:165640905..165640924 60.43 60
downstream ENSMUSE00000679649 Chr2:165640817..165640969 GACTCGCTCAGCTCCTTCAG Chr2:165640905..165640924 60.43 60
downstream ENSMUSE00000596214 Chr2:165637849..165638358 CACTGTGGGAGAATCCGTTT Chr2:165637948..165637967 59.97 50
downstream ENSMUSE00000639157 Chr2:165637849..165638358 CACTGTGGGAGAATCCGTTT Chr2:165637948..165637967 59.97 50
downstream ENSMUSE00000592802 Chr2:165633175..165633429 ACGGTAGACATCGTGCTGGT Chr2:165633266..165633285 60.6 55
downstream ENSMUSE00000550279 Chr2:165633019..165633429 ACTTTTGGGAGGACGTTTGA Chr2:165633126..165633145 59.57 45
downstream ENSMUSE00000679647 Chr2:165633019..165633429 ACTTTTGGGAGGACGTTTGA Chr2:165633126..165633145 59.57 45
downstream ENSMUSE00000639156 Chr2:165630687..165630877 CTCTGGGTGACCTCCTTCTG Chr2:165630828..165630847 59.83 60
downstream ENSMUSE00000679646 Chr2:165630687..165630877 CTCTGGGTGACCTCCTTCTG Chr2:165630828..165630847 59.83 60
downstream ENSMUSE00000635218 Chr2:165623434..165623514 GCTATTGTGCTCCCAGTGGT Chr2:165623416..165623435 60.14 55
downstream ENSMUSE00000639155 Chr2:165623434..165623514 GCTATTGTGCTCCCAGTGGT Chr2:165623416..165623435 60.14 55
downstream ENSMUSE00000639154 Chr2:165621583..165621661 ATCTCAATCCTCAGCCTTCG Chr2:165621617..165621636 59.39 50
downstream ENSMUSE00000679643 Chr2:165621583..165621661 ATCTCAATCCTCAGCCTTCG Chr2:165621617..165621636 59.39 50
downstream ENSMUSE00000635216 Chr2:165619895..165620134 CCAGCTCCAGTTGCTTTTTC Chr2:165620029..165620048 59.99 50
downstream ENSMUSE00000639153 Chr2:165619895..165620134 CCAGCTCCAGTTGCTTTTTC Chr2:165620029..165620048 59.99 50
downstream ENSMUSE00000679674 Chr2:165618312..165618467 GTTGCCCTGCGATGACTTAT Chr2:165618375..165618394 60.1 50
downstream ENSMUSE00000639152 Chr2:165618304..165618467 GTTGCCCTGCGATGACTTAT Chr2:165618375..165618394 60.1 50
downstream ENSMUSE00000679641 Chr2:165618304..165618467 GTTGCCCTGCGATGACTTAT Chr2:165618375..165618394 60.1 50
downstream ENSMUSE00000639151 Chr2:165617405..165617471 No primer for this exon
downstream ENSMUSE00000679640 Chr2:165617405..165617471 No primer for this exon
downstream ENSMUSE00000679672 Chr2:165617405..165617471 No primer for this exon
downstream ENSMUSE00000639150 Chr2:165612664..165612747 GGCTGGTGGTCTGTAGTGGT Chr2:165612668..165612687 60.03 60
downstream ENSMUSE00000679639 Chr2:165612664..165612747 GGCTGGTGGTCTGTAGTGGT Chr2:165612668..165612687 60.03 60
downstream ENSMUSE00000639147 Chr2:165611019..165611238 GCTCACAATGCACCCCTAGT Chr2:165611053..165611072 60.14 55
downstream ENSMUSE00000679638 Chr2:165610811..165611238 GCTCACAATGCACCCCTAGT Chr2:165611053..165611072 60.14 55
downstream ENSMUSE00000706073 Chr2:165610811..165611238 GCTCACAATGCACCCCTAGT Chr2:165611053..165611072 60.14 55
downstream ENSMUSE00000679680 Chr2:165610809..165611238 GCTCACAATGCACCCCTAGT Chr2:165611053..165611072 60.14 55
downstream ENSMUSE00000420365 Chr2:165610782..165611238 GCTCACAATGCACCCCTAGT Chr2:165611053..165611072 60.14 55
downstream ENSMUSE00000639169 Chr2:165609655..165611238 GGGCCTACGTGAAAATAGCA Chr2:165610625..165610644 60.1 50
downstream ENSMUSE00000639168 Chr2:165609654..165611238 GGGCCTACGTGAAAATAGCA Chr2:165610625..165610644 60.1 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GAGGTGGTGGAGGGAATGTA Chr2:165701223..165701243 59.78 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GAATGCGTGACTGGGAAAAC Chr2:165701210..165701230 60.5 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000039671