Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI15770
Trapped Gene
Btk (ENSMUSG00000031264)
Vector Insertion
Chr X: 131117549 - 131117656
Public Clones not available
Private Clones OST500471 (lexicon) OST473245 (lexicon) OST473237 (lexicon) OST473221 (lexicon)
OST473218 (lexicon) OST473207 (lexicon) OST472728 (lexicon) OST472638 (lexicon)
OST472603 (lexicon) OST472232 (lexicon) OST471965 (lexicon) OST471940 (lexicon)
OST471924 (lexicon) OST471904 (lexicon) OST471867 (lexicon) OST471856 (lexicon)
OST471840 (lexicon) OST471837 (lexicon) OST471828 (lexicon) OST471824 (lexicon)
OST471744 (lexicon) OST471724 (lexicon) OST471716 (lexicon) OST471678 (lexicon)
OST471631 (lexicon) OST471354 (lexicon) OST471353 (lexicon) OST471327 (lexicon)
OST471190 (lexicon) OST471136 (lexicon) OST470993 (lexicon) OST470940 (lexicon)
OST470894 (lexicon) OST470861 (lexicon) OST470821 (lexicon) OST470791 (lexicon)
OST470518 (lexicon) OST470353 (lexicon) OST470276 (lexicon) OST470194 (lexicon)
OST469928 (lexicon) OST469401 (lexicon) OST469400 (lexicon) OST469273 (lexicon)
OST468723 (lexicon) OST468079 (lexicon) OST467944 (lexicon) OST467847 (lexicon)
OST467835 (lexicon) OST467829 (lexicon) OST467825 (lexicon) OST467814 (lexicon)
OST467806 (lexicon) OST467791 (lexicon) OST467774 (lexicon) OST467761 (lexicon)
OST467740 (lexicon) OST467677 (lexicon) OST467430 (lexicon) OST467413 (lexicon)
OST467177 (lexicon) OST467175 (lexicon) OST467159 (lexicon) OST467109 (lexicon)
OST467094 (lexicon) OST466916 (lexicon) OST466901 (lexicon) OST466870 (lexicon)
OST466786 (lexicon) OST466781 (lexicon) OST466427 (lexicon) OST466375 (lexicon)
OST466317 (lexicon) OST466286 (lexicon) OST466276 (lexicon) OST466072 (lexicon)
OST466069 (lexicon) OST465885 (lexicon) OST465772 (lexicon) OST465751 (lexicon)
OST465710 (lexicon) OST465585 (lexicon) OST465565 (lexicon) OST465464 (lexicon)
OST465343 (lexicon) OST465342 (lexicon) OST465310 (lexicon) OST465263 (lexicon)
OST465254 (lexicon) OST465147 (lexicon) OST465134 (lexicon) OST465133 (lexicon)
OST465031 (lexicon) OST464847 (lexicon) OST464805 (lexicon) OST464713 (lexicon)
OST464707 (lexicon) OST464678 (lexicon) OST464574 (lexicon) OST464502 (lexicon)
OST464479 (lexicon) OST464463 (lexicon) OST464432 (lexicon) OST464431 (lexicon)
OST464423 (lexicon) OST464401 (lexicon) OST464377 (lexicon) OST464355 (lexicon)
OST464350 (lexicon) OST464349 (lexicon) OST464297 (lexicon) OST464292 (lexicon)
OST464254 (lexicon) OST463981 (lexicon) OST463786 (lexicon) OST463143 (lexicon)
OST463082 (lexicon) OST462847 (lexicon) OST462710 (lexicon) OST462543 (lexicon)
OST462370 (lexicon) OST462210 (lexicon) OST461804 (lexicon) OST461686 (lexicon)
OST461622 (lexicon) OST461499 (lexicon) OST461393 (lexicon) OST461207 (lexicon)
OST461151 (lexicon) OST461130 (lexicon) OST461058 (lexicon) OST461025 (lexicon)
OST460823 (lexicon) OST460773 (lexicon) OST460188 (lexicon) OST460141 (lexicon)
OST459845 (lexicon) OST459519 (lexicon) OST459494 (lexicon) OST459462 (lexicon)
OST459252 (lexicon) OST459239 (lexicon) OST459084 (lexicon) OST459054 (lexicon)
OST458964 (lexicon) OST458791 (lexicon) OST458637 (lexicon) OST458503 (lexicon)
OST458491 (lexicon) OST458215 (lexicon) OST458174 (lexicon) OST457840 (lexicon)
OST457746 (lexicon) OST457740 (lexicon) OST457379 (lexicon) OST457314 (lexicon)
OST457074 (lexicon) OST456897 (lexicon) OST456785 (lexicon) OST456726 (lexicon)
OST456701 (lexicon) OST456648 (lexicon) OST456636 (lexicon) OST456620 (lexicon)
OST174168 (lexicon) OST174142 (lexicon) OST174117 (lexicon) OST174044 (lexicon)
OST173973 (lexicon) OST173766 (lexicon) OST173675 (lexicon) OST173642 (lexicon)
OST173625 (lexicon) OST173614 (lexicon) OST173396 (lexicon) OST173395 (lexicon)
OST173356 (lexicon) OST173344 (lexicon) OST172891 (lexicon) OST172871 (lexicon)
OST172851 (lexicon) OST172639 (lexicon) OST172511 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000694799 (ChrX:131117550..131117667 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CTGGGGAGCTACCTGCATTA ChrX:131117563..131117582 60.23 55 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000694799 (ChrX:131117550..131117667 -)
Downstram Exon
ENSMUSE00000622602 (ChrX:131117550..131117655 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CTGGGGAGCTACCTGCATTA ChrX:131117563..131117582 60.23 55 GTCGTGAGATGCCAGGACTT ChrX:131117563..131117582 60.27 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000622602 ChrX:131117550..131117655 CTGGGGAGCTACCTGCATTA ChrX:131117563..131117582 60.23 55
upstream ENSMUSE00000694799 ChrX:131117550..131117667 CTGGGGAGCTACCTGCATTA ChrX:131117563..131117582 60.23 55

*** Putative Vector Insertion (Chr X: 131117549 - 131117656) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000694798 ChrX:131112009..131112163 No primer for this exon
downstream ENSMUSE00000277353 ChrX:131108456..131108626 TCAAGAGAAACAGGCGCTTC ChrX:131108478..131108497 60.66 50
downstream ENSMUSE00000712195 ChrX:131108456..131108626 TCAAGAGAAACAGGCGCTTC ChrX:131108478..131108497 60.66 50
downstream ENSMUSE00000207746 ChrX:131107748..131107846 CTTTCTGGTGGGGGATTTTT ChrX:131107736..131107755 60.16 45
downstream ENSMUSE00000207739 ChrX:131103444..131103512 AATGGGTACGGGAACCTTTC ChrX:131103426..131103445 60.05 50
downstream ENSMUSE00000207741 ChrX:131100136..131100217 GCTCTTCAGTTGGGGAGAAA ChrX:131100147..131100166 59.4 50
downstream ENSMUSE00000277330 ChrX:131093737..131093865 CAAAATTTGGCAGCCCATAG ChrX:131093731..131093750 60.45 45
downstream ENSMUSE00000277322 ChrX:131093226..131093293 GGGAAGAGGCTTTTTCGTTT ChrX:131093225..131093244 59.7 45
downstream ENSMUSE00000207752 ChrX:131091327..131091514 GACCTTTTTCAGCTCGGTTG ChrX:131091424..131091443 59.85 50
downstream ENSMUSE00000207745 ChrX:131090877..131090939 CTTGGGATGTAGCCTTCCTG ChrX:131090897..131090916 59.69 55
downstream ENSMUSE00000207740 ChrX:131090088..131090142 TGACTTCGAGTCATGTGCTTG ChrX:131090091..131090111 60.04 47.62
downstream ENSMUSE00000207736 ChrX:131089228..131089307 CCAGCTTTGCTGGAGTCTCT ChrX:131089242..131089261 59.74 55
downstream ENSMUSE00000694803 ChrX:131089002..131089065 GCGTGGAACACACAACGTAA ChrX:131089000..131089019 60.62 50
downstream ENSMUSE00000207748 ChrX:131088938..131089065 GCGTGGAACACACAACGTAA ChrX:131089000..131089019 60.62 50
downstream ENSMUSE00000207750 ChrX:131085741..131085815 TAGAAGGCGCGTTTTTGTTT ChrX:131085737..131085756 59.89 40
downstream ENSMUSE00000207751 ChrX:131084846..131085017 ATTCATCCTCCGACATGGAA ChrX:131084849..131084868 60.28 45
downstream ENSMUSE00000207743 ChrX:131083954..131084170 AAGGAACTGCTTCGACTCCA ChrX:131083944..131083963 59.99 50
downstream ENSMUSE00000207753 ChrX:131082101..131082165 No primer for this exon
downstream ENSMUSE00000207742 ChrX:131081251..131081369 TTGGAGCCTACAGAGCTGGT ChrX:131081306..131081325 60.01 55
downstream ENSMUSE00000207747 ChrX:131080021..131080178 ATGCCAGATGAGGCCTGTAG ChrX:131080040..131080059 60.24 55
downstream ENSMUSE00000694802 ChrX:131076980..131077346 GTATGCCAGGGAACGAGAGA ChrX:131077086..131077105 60.22 55
downstream ENSMUSE00000368789 ChrX:131076914..131077346 GTATGCCAGGGAACGAGAGA ChrX:131077086..131077105 60.22 55
downstream ENSMUSE00000694797 ChrX:131076909..131077346 GTATGCCAGGGAACGAGAGA ChrX:131077086..131077105 60.22 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGGTTTGCTCAGACTGTCCT ChrX:131117668..131117688 59.31 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CAAGTCCCACCTTCGTGACT ChrX:131117598..131117618 60.15 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000031264