Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI15775
Trapped Gene
Pgs1 (ENSMUSG00000017715)
Vector Insertion
Chr 11: 117876037 - 117880920
Public Clones not available
Private Clones OST500052 (lexicon) OST449193 (lexicon) OST422899 (lexicon) OST422593 (lexicon)
OST364070 (lexicon) OST350791 (lexicon) OST202984 (lexicon) OST70864 (lexicon)
OST43430 (lexicon) OST39581 (lexicon)
Severity of mutation (?) Insertion after 93% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000669467 (Chr11:117875961..117876036 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000669467 (Chr11:117875961..117876036 +)
Downstram Exon
ENSMUSE00000669466 (Chr11:117880921..117881046 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000427949 Chr11:117848171..117848327 No primer for this exon
upstream ENSMUSE00000367710 Chr11:117858755..117858944 No primer for this exon
upstream ENSMUSE00000151763 Chr11:117861513..117861590 No primer for this exon
upstream ENSMUSE00000669468 Chr11:117862336..117862347 No primer for this exon
upstream ENSMUSE00000151762 Chr11:117862930..117863029 No primer for this exon
upstream ENSMUSE00000151767 Chr11:117863665..117863854 No primer for this exon
upstream ENSMUSE00000151764 Chr11:117864655..117864833 No primer for this exon
upstream ENSMUSE00000360045 Chr11:117866684..117867205 No primer for this exon
upstream ENSMUSE00000398350 Chr11:117875888..117876036 No primer for this exon
upstream ENSMUSE00000669467 Chr11:117875961..117876036 No primer for this exon

*** Putative Vector Insertion (Chr 11: 117876037 - 117880920) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000346452 Chr11:117880921..117881046 No primer for this exon
downstream ENSMUSE00000669466 Chr11:117880921..117881046 No primer for this exon
downstream ENSMUSE00000384302 Chr11:117884719..117885325 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GTTGGAGCGGTAGGGTTCTT Chr11:117879042..117879062 60.5 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GCTGTCCGGTTTTATCGTGA Chr11:117879073..117879093 61.03 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000017715