Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI15781
Trapped Gene
Prtg (ENSMUSG00000036030)
Vector Insertion
Chr 9: 72758549 - 72759710
Public Clones IST14958C3 (tigm)
Private Clones OST499937 (lexicon) OST14921 (lexicon) OST12391 (lexicon)
Severity of mutation (?) Insertion after 89% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000387136 (Chr9:72758444..72758548 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000387136 (Chr9:72758444..72758548 +)
Downstram Exon
ENSMUSE00000352082 (Chr9:72759711..72761965 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon TAAAAGCAGACGGCCTGAGT Chr9:72759748..72759767 60.01 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000635590 Chr9:72655018..72655339 CTGCTGCTGTTGCTGCTACT Chr9:72655297..72655316 59.56 55
upstream ENSMUSE00000635589 Chr9:72657262..72657564 CAAATACGGGGCCATTCTTA Chr9:72657518..72657537 59.79 45
upstream ENSMUSE00000309286 Chr9:72690502..72690646 GCGTTTGAAGTCCATCCAGT Chr9:72690510..72690529 60.12 50
upstream ENSMUSE00000309264 Chr9:72692665..72692798 CTGGGAATTACCGCTGTGTT Chr9:72692721..72692740 59.99 50
upstream ENSMUSE00000407802 Chr9:72695716..72695853 AGGATATCCCAGACCCATCA Chr9:72695816..72695835 59.17 50
upstream ENSMUSE00000309215 Chr9:72696121..72696279 ACTTGGAAATGGCAACCTCA Chr9:72696155..72696174 60.49 45
upstream ENSMUSE00000309192 Chr9:72697559..72697718 GGAATTCCCTCACCCAAAAT Chr9:72697639..72697658 60 45
upstream ENSMUSE00000309167 Chr9:72699288..72699535 CAGATTATCCCGGAAGACGA Chr9:72699304..72699323 60.03 50
upstream ENSMUSE00000309138 Chr9:72702763..72702927 CTTGGAAACGACACGACTCA Chr9:72702792..72702811 59.87 50
upstream ENSMUSE00000389884 Chr9:72704582..72704887 TTACTGCAGCTACCCGTGTG Chr9:72704807..72704826 59.93 55
upstream ENSMUSE00000332741 Chr9:72706568..72706756 CTATTCGGGGCTACAAGCTG Chr9:72706655..72706674 59.86 55
upstream ENSMUSE00000375893 Chr9:72738584..72738679 AATACCACGTGAGGCTCCTG Chr9:72738596..72738615 60.13 55
upstream ENSMUSE00000340670 Chr9:72739616..72739802 CTCTACGCGAAGGCGAATAC Chr9:72739660..72739679 60 55
upstream ENSMUSE00000383878 Chr9:72740035..72740162 AGTCCCTGGAGTCCTGTGGT Chr9:72740120..72740139 60.97 60
upstream ENSMUSE00000347876 Chr9:72741631..72741801 GGGGTAAAGGTGACCCTGAT Chr9:72741651..72741670 60.05 55
upstream ENSMUSE00000393523 Chr9:72752512..72752694 ATATCTGCGTCCAACGAGGT Chr9:72752571..72752590 59.58 50
upstream ENSMUSE00000338515 Chr9:72753955..72754075 AAAAGTCAATGACGGGCATC Chr9:72753982..72754001 59.94 45
upstream ENSMUSE00000384050 Chr9:72755508..72755670 TAAGACAGCGCAGAGTGGAA Chr9:72755523..72755542 59.74 50
upstream ENSMUSE00000387136 Chr9:72758444..72758548 No primer for this exon

*** Putative Vector Insertion (Chr 9: 72758549 - 72759710) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000352082 Chr9:72759711..72761965 TAAAAGCAGACGGCCTGAGT Chr9:72759748..72759767 60.01 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CACCCATGTGAGGAGAATCC Chr9:72758556..72758576 60.33 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CACCCATGTGAGGAGAATCC Chr9:72758556..72758576 60.33 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000036030