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UniTrap UNI15836
Trapped Gene
Casc5 (ENSMUSG00000027326)
Vector Insertion
Chr 2: 118873297 - 118883016
Public Clones E122B10 (ggtc) E122B10 (ggtc) IST14231B2 (tigm)
Private Clones OST471984 (lexicon) OST470183 (lexicon) OST434038 (lexicon) OST246134 (lexicon)
OST132967 (lexicon) OST103099 (lexicon) OST91970 (lexicon) OST68407 (lexicon)
OST57673 (lexicon) OST48113 (lexicon) OST43139 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000345237 (Chr2:118872886..118873296 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GAATGCTCCTTTCCGTTCTG Chr2:118872978..118872997 59.81 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000345237 (Chr2:118872886..118873296 +)
Downstram Exon
ENSMUSE00000708026 (Chr2:118883017..118883067 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GAATGCTCCTTTCCGTTCTG Chr2:118872978..118872997 59.81 50 GCTTCTGAATATACCCCATCCA Chr2:118883056..118883077 60.17 45.46

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000685609 Chr2:118872855..118872959 GCATCACTAGGGCGGTTAGA Chr2:118872866..118872885 60.24 55
upstream ENSMUSE00000345237 Chr2:118872886..118873296 GAATGCTCCTTTCCGTTCTG Chr2:118872978..118872997 59.81 50

*** Putative Vector Insertion (Chr 2: 118873297 - 118883016) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000384970 Chr2:118883017..118883067 GCTTCTGAATATACCCCATCCA Chr2:118883056..118883077 60.17 45.46
downstream ENSMUSE00000708026 Chr2:118883017..118883067 GCTTCTGAATATACCCCATCCA Chr2:118883056..118883077 60.17 45.46
downstream ENSMUSE00000168443 Chr2:118884091..118884130 No primer for this exon
downstream ENSMUSE00000168435 Chr2:118885280..118885339 TCCTGAAGAGGACTCCTTGG Chr2:118885314..118885333 59.38 55
downstream ENSMUSE00000168441 Chr2:118888096..118888157 GACTCGACGAGAGCTTTTCC Chr2:118888140..118888159 59.17 55
downstream ENSMUSE00000168434 Chr2:118889781..118889833 No primer for this exon
downstream ENSMUSE00000168433 Chr2:118891685..118891737 CTGAATTGGAGCACAAAGCA Chr2:118891719..118891738 59.99 45
downstream ENSMUSE00000168437 Chr2:118893859..118898655 AGCGCTATGGCACTTTCAGT Chr2:118897217..118897236 60.04 50
downstream ENSMUSE00000642270 Chr2:118893859..118898355 AGCGCTATGGCACTTTCAGT Chr2:118897217..118897236 60.04 50
downstream ENSMUSE00000642269 Chr2:118901968..118902106 TGCTGCAACTAGACGGTGTC Chr2:118902061..118902080 60.06 55
downstream ENSMUSE00000642268 Chr2:118902672..118902739 No primer for this exon
downstream ENSMUSE00000642267 Chr2:118903634..118903732 GGAGAGTGCTCTGCCTAGGTT Chr2:118903726..118903746 60.03 57.14
downstream ENSMUSE00000642266 Chr2:118907246..118907361 TCTTTTGTCGAAGAAGCTCACA Chr2:118907355..118907376 60.17 40.91
downstream ENSMUSE00000642265 Chr2:118912296..118912386 TCACTTTTTCCCACAAGTTCCT Chr2:118912367..118912388 60.01 40.91
downstream ENSMUSE00000642264 Chr2:118913212..118913328 GAGCCACTTTTCCTTGGTGA Chr2:118913302..118913321 60.23 50
downstream ENSMUSE00000168450 Chr2:118914535..118914622 TTTCCACTTCAGCGAGACAG Chr2:118914622..118914641 59.16 50
downstream ENSMUSE00000168463 Chr2:118919742..118919819 CATTCTTCCATGGCATCATTT Chr2:118919792..118919812 59.78 38.1
downstream ENSMUSE00000168458 Chr2:118920002..118920041 No primer for this exon
downstream ENSMUSE00000168456 Chr2:118920864..118920965 AGGGTCTGCGTTTTCTGTGT Chr2:118920905..118920924 59.77 50
downstream ENSMUSE00000168454 Chr2:118923154..118923254 GGCTCTCCAAAGGTGATGAT Chr2:118923252..118923271 59.09 50
downstream ENSMUSE00000168448 Chr2:118925498..118925566 No primer for this exon
downstream ENSMUSE00000168452 Chr2:118926449..118926558 GCTGTGCTGTGCATTTCTTC Chr2:118926547..118926566 59.6 50
downstream ENSMUSE00000168447 Chr2:118927718..118927836 GCTATAATTTGGCCCCCATC Chr2:118927810..118927829 60.49 50
downstream ENSMUSE00000168462 Chr2:118928235..118928357 CAAATGCTGCACAACTGGAG Chr2:118928272..118928291 60.45 50
downstream ENSMUSE00000168460 Chr2:118929714..118929857 CTGTGGGTCTTACGGGAAAA Chr2:118929839..118929858 59.96 50
downstream ENSMUSE00000685604 Chr2:118929714..118931237 CTGTGGGTCTTACGGGAAAA Chr2:118929839..118929858 59.96 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CTTAATCGCCTTGCAGCACA Chr2:118873346..118873366 62.38 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AACTCGTGACTGGGAAAACC Chr2:118873344..118873364 59.04 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000027326