Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI15845
Trapped Gene
Narfl (ENSMUSG00000002280)
Vector Insertion
Chr 17: 25912087 - 25913910
Public Clones not available
Private Clones OST471845 (lexicon)
Severity of mutation (?) Insertion after 11% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000440524 (Chr17:25911991..25912086 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000440524 (Chr17:25911991..25912086 +)
Downstram Exon
ENSMUSE00000152768 (Chr17:25913911..25914054 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000395567 Chr17:25910745..25910832 No primer for this exon
upstream ENSMUSE00000440524 Chr17:25911991..25912086 No primer for this exon

*** Putative Vector Insertion (Chr 17: 25912087 - 25913910) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000152768 Chr17:25913911..25914054 No primer for this exon
downstream ENSMUSE00000152770 Chr17:25914270..25914402 No primer for this exon
downstream ENSMUSE00000152777 Chr17:25915895..25916029 No primer for this exon
downstream ENSMUSE00000152774 Chr17:25916443..25916561 No primer for this exon
downstream ENSMUSE00000152775 Chr17:25917252..25917381 No primer for this exon
downstream ENSMUSE00000287325 Chr17:25917825..25917897 No primer for this exon
downstream ENSMUSE00000152772 Chr17:25918186..25918323 No primer for this exon
downstream ENSMUSE00000152776 Chr17:25918633..25918790 No primer for this exon
downstream ENSMUSE00000152773 Chr17:25918961..25920277 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
  No PCR available

Come back to gene ENSMUSG00000002280