Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI15860
Trapped Gene
Acsbg2 (ENSMUSG00000024207)
Vector Insertion
Chr 17: 56982801 - 56984826
Public Clones not available
Private Clones OST471429 (lexicon) OST465166 (lexicon) OST441661 (lexicon) OST441654 (lexicon)
OST319436 (lexicon) OST316236 (lexicon) OST56709 (lexicon) OST27216 (lexicon)
OST18379 (lexicon)
Severity of mutation (?) Insertion after 100% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000139733 (Chr17:56984827..56984936 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000139733 (Chr17:56984827..56984936 -)
Downstram Exon
ENSMUSE00000606648 (Chr17:56982527..56982800 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon AGGAAGGTGTTTTGGGCTCT Chr17:56982548..56982567 60.11 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000606654 Chr17:57013620..57013870 CTGGCCACCTTGTGAAAGTT Chr17:57013621..57013640 60.15 50
upstream ENSMUSE00000606653 Chr17:57012220..57012320 GAGAAGAAGGCGGAAGACCT Chr17:57012246..57012265 59.96 55
upstream ENSMUSE00000606652 Chr17:57007649..57007878 CCTCAAAGAACGGCAAAAAG Chr17:57007715..57007734 59.85 45
upstream ENSMUSE00000606651 Chr17:57003883..57003971 GGTCGAGTGGGTCGTTACTG Chr17:57003905..57003924 60.57 60
upstream ENSMUSE00000606650 Chr17:57002294..57002414 GCAGGCCAACGTGAGTATCT Chr17:57002335..57002354 60.28 55
upstream ENSMUSE00000693748 Chr17:57002290..57002414 GCAGGCCAACGTGAGTATCT Chr17:57002335..57002354 60.28 55
upstream ENSMUSE00000693747 Chr17:57002084..57002091 No primer for this exon
upstream ENSMUSE00000606649 Chr17:57000962..57001045 GAGACCGTGAAAGCCATTGT Chr17:57001008..57001027 60.12 50
upstream ENSMUSE00000693746 Chr17:57000962..57001030 GAGACCGTGAAAGCCATTGT Chr17:57001008..57001027 60.12 50
upstream ENSMUSE00000463791 Chr17:56996302..56996451 CCAACCAGTGTGCTGTCATC Chr17:56996359..56996378 60.16 55
upstream ENSMUSE00000139734 Chr17:56993160..56993336 AGACATCTGGATCCCCATCA Chr17:56993204..56993223 60.29 50
upstream ENSMUSE00000139739 Chr17:56989458..56989639 CCTTGGTGTACACGCTTCAA Chr17:56989616..56989635 59.76 50
upstream ENSMUSE00000139742 Chr17:56989138..56989371 GACATCCCCATGAATTACCG Chr17:56989345..56989364 60.01 50
upstream ENSMUSE00000139735 Chr17:56987034..56987251 TATATCACTGGCCGCATCAA Chr17:56987036..56987055 60.06 45
upstream ENSMUSE00000139740 Chr17:56985945..56986084 AATCCTGATAACCGCTGGTG Chr17:56986064..56986083 59.96 50
upstream ENSMUSE00000139736 Chr17:56985516..56985762 AAGTGGTCTACACGGCCATC Chr17:56985622..56985641 60 55
upstream ENSMUSE00000139733 Chr17:56984827..56984936 No primer for this exon

*** Putative Vector Insertion (Chr 17: 56982801 - 56984826) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000606648 Chr17:56982527..56982800 AGGAAGGTGTTTTGGGCTCT Chr17:56982548..56982567 60.11 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGTATCCTTAATCGCCTTGC Chr17:56984763..56984783 58.67 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ATCCTCGTGACTGGGAAAAC Chr17:56984760..56984780 58.99 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000024207