Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI15877
Trapped Gene
Nhsl1 (ENSMUSG00000039835)
Vector Insertion
Chr 10: 18189972 - 18192724
Public Clones not available
Private Clones OST471184 (lexicon) OST375229 (lexicon) OST320731 (lexicon) OST274655 (lexicon)
OST234413 (lexicon) OST35812 (lexicon)
Severity of mutation (?) Insertion after 4% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000577217 (Chr10:18189914..18189971 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000577217 (Chr10:18189914..18189971 +)
Downstram Exon
ENSMUSE00000320434 (Chr10:18192725..18192877 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon TCGACTCTCCTCGTCCAAGT Chr10:18192756..18192775 59.99 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000644792 Chr10:18127481..18128320 GACGCTGGATCTTTTTCTGC Chr10:18127638..18127657 59.96 50
upstream ENSMUSE00000577217 Chr10:18189914..18189971 No primer for this exon

*** Putative Vector Insertion (Chr 10: 18189972 - 18192724) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000320434 Chr10:18192725..18192877 TCGACTCTCCTCGTCCAAGT Chr10:18192756..18192775 59.99 55
downstream ENSMUSE00000421066 Chr10:18217839..18217963 TTGCCTCAGCTTGTCACATT Chr10:18217861..18217880 59.44 45
downstream ENSMUSE00000410215 Chr10:18231356..18231548 TGAGGTTGGCGTTTTAGGTC Chr10:18231424..18231443 60.11 50
downstream ENSMUSE00000363998 Chr10:18235835..18235966 CCTGACGATCGAAATTCTCC Chr10:18235858..18235877 59.63 50
downstream ENSMUSE00000710484 Chr10:18243597..18246848 GAGAGGGGCTAACTGCTGTG Chr10:18245909..18245928 60.01 60
downstream ENSMUSE00000713881 Chr10:18243597..18246848 GAGAGGGGCTAACTGCTGTG Chr10:18245909..18245928 60.01 60
downstream ENSMUSE00000320342 Chr10:18247342..18247474 ATACATCCACGGGGTCTGAA Chr10:18247398..18247417 60.19 50
downstream ENSMUSE00000666582 Chr10:18247342..18247474 ATACATCCACGGGGTCTGAA Chr10:18247398..18247417 60.19 50
downstream ENSMUSE00000644788 Chr10:18251074..18253695 CACGATGCCTCTCTTCCTTC Chr10:18252569..18252588 59.95 55
downstream ENSMUSE00000666581 Chr10:18251074..18253695 CACGATGCCTCTCTTCCTTC Chr10:18252569..18252588 59.95 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TAATCGCCTTGCAGCACATC Chr10:18190023..18190043 62.25 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector

Come back to gene ENSMUSG00000039835