Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI15891
Trapped Gene
Taf12 (ENSMUSG00000028899)
Vector Insertion
Chr 4: 131837994 - 131838708
Public Clones P145C09 (ggtc) P145C09 (ggtc)
Private Clones OST470888 (lexicon) OST464800 (lexicon) OST378244 (lexicon) OST355789 (lexicon)
OST334491 (lexicon) OST333545 (lexicon) OST309688 (lexicon) OST302805 (lexicon)
OST302183 (lexicon) OST254305 (lexicon) OST236996 (lexicon) OST180421 (lexicon)
OST165169 (lexicon) OST147781 (lexicon) OST143934 (lexicon) OST136264 (lexicon)
OST125674 (lexicon) OST125587 (lexicon) OST117570 (lexicon) OST48476 (lexicon)
OST42853 (lexicon) OST24377 (lexicon) OST23236 (lexicon) OST16023 (lexicon)
OST15446 (lexicon) OST6616 (lexicon) OST5004 (lexicon) OST1636 (lexicon)
OST1150 (lexicon)
Severity of mutation (?) Insertion after 11% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000668043 (Chr4:131837909..131837993 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CAGTGGGCTTACAGCAGTTG Chr4:131837939..131837958 59.51 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000668043 (Chr4:131837909..131837993 +)
Downstram Exon
ENSMUSE00000668042 (Chr4:131838709..131838960 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CAGTGGGCTTACAGCAGTTG Chr4:131837939..131837958 59.51 55 GTTTTCAGGGCTGAGACGAC Chr4:131838957..131838976 59.85 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000414762 Chr4:131830328..131830402 CTGCTGAGACGAAAGCTTCA Chr4:131830338..131830357 59.47 50
upstream ENSMUSE00000668044 Chr4:131830331..131830402 CTGCTGAGACGAAAGCTTCA Chr4:131830338..131830357 59.47 50
upstream ENSMUSE00000668043 Chr4:131837909..131837993 CAGTGGGCTTACAGCAGTTG Chr4:131837939..131837958 59.51 55

*** Putative Vector Insertion (Chr 4: 131837994 - 131838708) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000183257 Chr4:131838709..131838960 GTTTTCAGGGCTGAGACGAC Chr4:131838957..131838976 59.85 55
downstream ENSMUSE00000668042 Chr4:131838709..131838960 GTTTTCAGGGCTGAGACGAC Chr4:131838957..131838976 59.85 55
downstream ENSMUSE00000381198 Chr4:131840959..131841036 TTCATCCAGCTGCTCATTAGG Chr4:131841026..131841046 60.36 47.62
downstream ENSMUSE00000338088 Chr4:131845230..131845344 GCAGCTGGACGTCTTTTACC Chr4:131845341..131845360 59.88 55
downstream ENSMUSE00000373585 Chr4:131847778..131847866 TGAGCCTCTGTGGTACATGC Chr4:131847862..131847881 59.86 55
downstream ENSMUSE00000333148 Chr4:131848542..131849006 TGGTTGTTTTCCGGATCAGT Chr4:131848572..131848591 60.35 45
downstream ENSMUSE00000668041 Chr4:131848542..131848627 TGGTTGTTTTCCGGATCAGT Chr4:131848572..131848591 60.35 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGGCAGAACTTGGTGAATGT Chr4:131838021..131838041 59.97 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GGGCAGAACTTGGTGAATGT Chr4:131838021..131838041 59.97 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000028899