Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI15894
Trapped Gene
Gcnt2 (ENSMUSG00000021360)
Vector Insertion
Chr 13: 41014171 - 41048944
Public Clones PST24373-NR (escells) IST14256C4 (tigm)
Private Clones OST470859 (lexicon) OST469998 (lexicon) OST467906 (lexicon) OST464949 (lexicon)
OST464250 (lexicon) OST433812 (lexicon) OST411036 (lexicon) OST405978 (lexicon)
OST379093 (lexicon) OST367806 (lexicon) OST360968 (lexicon) OST360804 (lexicon)
OST357940 (lexicon) OST346807 (lexicon) OST326112 (lexicon) OST180688 (lexicon)
OST169399 (lexicon) OST169270 (lexicon) OST152753 (lexicon) OST149663 (lexicon)
OST147625 (lexicon) OST131216 (lexicon) OST97817 (lexicon) OST97613 (lexicon)
OST64770 (lexicon) OST59817 (lexicon) OST37918 (lexicon)
Severity of mutation (?) Insertion after 76% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000288319 (Chr13:41013003..41014170 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000288319 (Chr13:41013003..41014170 +)
Downstram Exon
ENSMUSE00000446426 (Chr13:41048945..41049040 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000683059 Chr13:40955501..40956642 No primer for this exon
upstream ENSMUSE00000500581 Chr13:40982535..40983654 No primer for this exon
upstream ENSMUSE00000683058 Chr13:40982736..40983658 No primer for this exon
upstream ENSMUSE00000683057 Chr13:40984564..40984579 No primer for this exon
upstream ENSMUSE00000288319 Chr13:41013003..41014170 No primer for this exon

*** Putative Vector Insertion (Chr 13: 41014171 - 41048944) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000446426 Chr13:41048945..41049040 No primer for this exon
downstream ENSMUSE00000446420 Chr13:41053521..41056260 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ACTCCGGATTTACTGGCATC Chr13:41041195..41041215 59.01 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TAACCCGTGACTGGGAAAAC Chr13:41041217..41041237 59.83 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000021360