Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI1596
Trapped Gene
Rexo4 (ENSMUSG00000052406)
Vector Insertion
Chr 2: 26818244 - 26819571
Public Clones CC0016 (sanger)
Private Clones not available
Severity of mutation (?) Insertion after 16% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000706960 (Chr2:26819572..26819832 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CAGGAAGCTCGCTAGGAAGA Chr2:26819706..26819725 59.85 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000706960 (Chr2:26819572..26819832 -)
Downstram Exon
ENSMUSE00000517801 (Chr2:26817903..26818243 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CAGGAAGCTCGCTAGGAAGA Chr2:26819706..26819725 59.85 55 CTTGAGAGCCCCTTGATGAG Chr2:26817937..26817956 59.94 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000232615 Chr2:26819572..26819906 CAGGAAGCTCGCTAGGAAGA Chr2:26819706..26819725 59.85 55
upstream ENSMUSE00000706960 Chr2:26819572..26819832 CAGGAAGCTCGCTAGGAAGA Chr2:26819706..26819725 59.85 55
upstream ENSMUSE00000706961 Chr2:26819572..26819838 CAGGAAGCTCGCTAGGAAGA Chr2:26819706..26819725 59.85 55

*** Putative Vector Insertion (Chr 2: 26818244 - 26819571) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000517801 Chr2:26817903..26818243 CTTGAGAGCCCCTTGATGAG Chr2:26817937..26817956 59.94 55
downstream ENSMUSE00000518782 Chr2:26816123..26816260 GGCCTATGGCATCCTCAATA Chr2:26816183..26816202 59.88 50
downstream ENSMUSE00000519992 Chr2:26815744..26815937 ACTGGTTCACGATCGACACA Chr2:26815821..26815840 60.16 50
downstream ENSMUSE00000232588 Chr2:26813999..26814087 CTGCCCTTCAACATTTCTGC Chr2:26814014..26814033 60.78 50
downstream ENSMUSE00000232582 Chr2:26811809..26811883 TCGACTCCTGAATGGTTTGA Chr2:26811793..26811812 59.22 45
downstream ENSMUSE00000232578 Chr2:26811015..26811089 GACCCTGATCCCCAGAATTT Chr2:26811014..26811033 60.13 50
downstream ENSMUSE00000697781 Chr2:26809086..26810116 AGAGTTCTTGATGCGGTGCT Chr2:26809978..26809997 60.02 50
downstream ENSMUSE00000232572 Chr2:26809083..26810116 AGAGTTCTTGATGCGGTGCT Chr2:26809978..26809997 60.02 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TCATCCCGAACTTTCTGTCA Chr2:26819527..26819547 59.22 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000052406