Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI1597
Trapped Gene
Hiat1 (ENSMUSG00000027958)
Vector Insertion
Chr 3: 116354418 - 116365312
Public Clones (sanger) (sanger) (sanger) (sanger) (sanger) CC0014 (sanger)
(sanger) P145H03 (ggtc) E039D09 (ggtc) M125G12 (ggtc) E045C11 (ggtc)
E045C11 (ggtc) FHCRC-GT-S21-8A1 (fhcrc) IST14376D9 (tigm) IST10951G11 (tigm)
IST15058F5 (tigm) IST14266B10 (tigm)
Private Clones OST459596 (lexicon) OST431602 (lexicon) OST412803 (lexicon) OST362463 (lexicon)
OST360958 (lexicon) OST358355 (lexicon) OST358143 (lexicon) OST351931 (lexicon)
OST333439 (lexicon) OST298204 (lexicon) OST276427 (lexicon) OST266648 (lexicon)
OST266351 (lexicon) OST246210 (lexicon) OST230403 (lexicon) OST220628 (lexicon)
OST217003 (lexicon) OST195166 (lexicon) OST146522 (lexicon) OST143233 (lexicon)
OST135678 (lexicon) OST128296 (lexicon) OST116283 (lexicon) OST112418 (lexicon)
OST86351 (lexicon)
Severity of mutation (?) Insertion after 6% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000357219 (Chr3:116365313..116365595 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TAAAATGACCCAGGGGAAGA Chr3:116365381..116365400 59.36 45 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000357219 (Chr3:116365313..116365595 -)
Downstram Exon
ENSMUSE00000324136 (Chr3:116354322..116354417 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TAAAATGACCCAGGGGAAGA Chr3:116365381..116365400 59.36 45 TGTCAGTAGTCCCCAAGCAA Chr3:116354315..116354334 59.29 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000357219 Chr3:116365313..116365595 TAAAATGACCCAGGGGAAGA Chr3:116365381..116365400 59.36 45

*** Putative Vector Insertion (Chr 3: 116354418 - 116365312) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000324136 Chr3:116354322..116354417 TGTCAGTAGTCCCCAAGCAA Chr3:116354315..116354334 59.29 50
downstream ENSMUSE00000324123 Chr3:116351437..116351499 TCCTTGAATTAGGCCATTCATC Chr3:116351421..116351442 60.28 40.91
downstream ENSMUSE00000324115 Chr3:116350700..116350827 GGCACACGTGAAAAACACTG Chr3:116350707..116350726 60.2 50
downstream ENSMUSE00000324106 Chr3:116348284..116348398 ACTGCAAAAACCCCAGAAAC Chr3:116348335..116348354 59.08 45
downstream ENSMUSE00000324096 Chr3:116344628..116344846 CCCATACACTCGTCCAAGGT Chr3:116344753..116344772 59.84 55
downstream ENSMUSE00000174661 Chr3:116344165..116344278 TATGGAGTCTTGGCCCACTT Chr3:116344227..116344246 59.55 50
downstream ENSMUSE00000324082 Chr3:116343305..116343376 ACAGAATGCCAAGGACTGCT Chr3:116343297..116343316 59.87 50
downstream ENSMUSE00000324072 Chr3:116339196..116339308 TCCCAGACCCAGTAAGATGG Chr3:116339221..116339240 59.92 55
downstream ENSMUSE00000324063 Chr3:116337821..116337918 TCAGCAGTCCGTGAAACAAG Chr3:116337813..116337832 60.03 50
downstream ENSMUSE00000174660 Chr3:116336640..116336803 CCAGGTCTGTCCCTGTGATT Chr3:116336653..116336672 59.96 55
downstream ENSMUSE00000401167 Chr3:116334105..116335398 ACGAGCAGGTAAAGGCTCAA Chr3:116334853..116334872 60.01 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TTTGGTAGGTGTGTGGTAGGC Chr3:116356272..116356293 59.91 52.38 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GATCCGTGACTGGGAAAACC Chr3:116356245..116356265 61.67 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 GTAATCGCCTTGCAGCACAT Chr3:116362526..116362546 61.2 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 ACGATGCGTGACTGGGAAAA Chr3:116362531..116362551 63.88 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000027958