Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI15971
Trapped Gene
Wtap (ENSMUSG00000060475)
Vector Insertion
Chr 17: 13176379 - 13178781
Public Clones (sanger) D044C06 (ggtc) D091F02 (ggtc) D044C06 (ggtc) D091F02 (ggtc)
PST19400-NR (escells) IST14193B6 (tigm)
Private Clones OST469150 (lexicon) OST456366 (lexicon) OST439624 (lexicon) OST383798 (lexicon)
OST297434 (lexicon) OST278071 (lexicon) OST272022 (lexicon) OST269584 (lexicon)
OST261228 (lexicon) OST218196 (lexicon) OST210061 (lexicon) OST198615 (lexicon)
OST181832 (lexicon) OST174671 (lexicon) OST133618 (lexicon) OST130985 (lexicon)
OST126481 (lexicon)
Severity of mutation (?) Insertion after 3% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000555871 (Chr17:13178782..13178819 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TGCAAGATGACCAACGAAGA Chr17:13178798..13178817 60.39 45 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000555871 (Chr17:13178782..13178819 -)
Downstram Exon
ENSMUSE00000243365 (Chr17:13176323..13176378 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TGCAAGATGACCAACGAAGA Chr17:13178798..13178817 60.39 45 ATCCCGTGCCATAACTTTGA Chr17:13176315..13176334 60.33 45

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000703761 Chr17:13184943..13185021 No primer for this exon
upstream ENSMUSE00000555871 Chr17:13178782..13178819 TGCAAGATGACCAACGAAGA Chr17:13178798..13178817 60.39 45

*** Putative Vector Insertion (Chr 17: 13176379 - 13178781) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000243365 Chr17:13176323..13176378 ATCCCGTGCCATAACTTTGA Chr17:13176315..13176334 60.33 45
downstream ENSMUSE00000135725 Chr17:13174616..13174674 TCCCTCCAAAGCTTGAACAT Chr17:13174613..13174632 59.67 45
downstream ENSMUSE00000135728 Chr17:13173688..13173815 TCTCCTGCTCTTTGGTTGCT Chr17:13173680..13173699 60.13 50
downstream ENSMUSE00000464217 Chr17:13168162..13168340 CATTTTGGGCTTGTTCCAGT Chr17:13168171..13168190 59.97 45
downstream ENSMUSE00000135730 Chr17:13162271..13162425 CATTCGACACTTCGCCATTA Chr17:13162367..13162386 59.69 45
downstream ENSMUSE00000483745 Chr17:13159669..13160917 ACTTGGTCCATTTGCCAGAC Chr17:13160669..13160688 59.97 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TTTCAGGGTGCAAGATGACC Chr17:13178804..13178824 61.05 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TGCAAGATGACCAACGAAGA Chr17:13178796..13178816 60.39 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 CCCCACCCCTTATTTTTCAG Chr17:13178818..13178838 60.53 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 CCCCACCCCTTATTTTTCAG Chr17:13178818..13178838 60.53 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000060475