Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI16181
Trapped Gene
Btf3l4 (ENSMUSG00000028568)
Vector Insertion
Chr 4: 108498819 - 108503122
Public Clones not available
Private Clones OST465545 (lexicon) OST429713 (lexicon) OST407670 (lexicon) OST395426 (lexicon)
OST293266 (lexicon) OST289918 (lexicon) OST268687 (lexicon) OST267415 (lexicon)
OST223785 (lexicon) OST215873 (lexicon) OST206615 (lexicon) OST183129 (lexicon)
OST150995 (lexicon) OST128995 (lexicon) OST122366 (lexicon) OST122346 (lexicon)
OST115614 (lexicon) OST98980 (lexicon) OST85691 (lexicon) OST83584 (lexicon)
OST65601 (lexicon) OST45595 (lexicon) OST44646 (lexicon) OST40616 (lexicon)
Severity of mutation (?) Insertion after 11% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000716151 (Chr4:108503123..108503188 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TCAAGCTCAGGTCCGGATAG Chr4:108503131..108503150 60.35 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000716151 (Chr4:108503123..108503188 -)
Downstram Exon
ENSMUSE00000525636 (Chr4:108498705..108498818 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TCAAGCTCAGGTCCGGATAG Chr4:108503131..108503150 60.35 55 TTCTTCTTCCTGCGAGCTGT Chr4:108498774..108498793 60.28 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000662460 Chr4:108506113..108506159 No primer for this exon
upstream ENSMUSE00000662466 Chr4:108506113..108506219 GAGGCAGCCATCTTGCTCTT Chr4:108506150..108506169 61.97 55
upstream ENSMUSE00000662461 Chr4:108505748..108506169 CAGTGTCAGACGCTTTGAGC Chr4:108505789..108505808 59.78 55
upstream ENSMUSE00000662459 Chr4:108505018..108505056 No primer for this exon
upstream ENSMUSE00000662457 Chr4:108504311..108504536 TGCTGCACAGCTAAGATTGG Chr4:108504469..108504488 60.16 50
upstream ENSMUSE00000662465 Chr4:108503123..108503188 TCAAGCTCAGGTCCGGATAG Chr4:108503131..108503150 60.35 55
upstream ENSMUSE00000716151 Chr4:108503123..108503188 TCAAGCTCAGGTCCGGATAG Chr4:108503131..108503150 60.35 55

*** Putative Vector Insertion (Chr 4: 108498819 - 108503122) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000525636 Chr4:108498705..108498818 TTCTTCTTCCTGCGAGCTGT Chr4:108498774..108498793 60.28 50
downstream ENSMUSE00000662464 Chr4:108491741..108491942 GCAAAGGTGTTAGCGGAGAG Chr4:108491838..108491857 60.01 55
downstream ENSMUSE00000662463 Chr4:108490790..108490849 CTGGTTTGGGCGCTTTACTA Chr4:108490801..108490820 60.26 50
downstream ENSMUSE00000662456 Chr4:108489437..108489491 GCTTCATTTTTCGATGCTTCA Chr4:108489430..108489450 60.34 38.1
downstream ENSMUSE00000662458 Chr4:108489396..108489491 TCTAGTCCATGCCAGCTTCC Chr4:108489388..108489407 60.36 55
downstream ENSMUSE00000601484 Chr4:108489323..108489491 TCTAGTCCATGCCAGCTTCC Chr4:108489388..108489407 60.36 55
downstream ENSMUSE00000662462 Chr4:108489055..108489491 CAAATGACCGAGAACTGCTG Chr4:108489264..108489283 59.44 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGTGATTGCCGGAGAGTGTC Chr4:108500098..108500118 60.27 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGCCAAACTTCAAGCTCAGG Chr4:108503138..108503158 59.62 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000028568