Tools for the biologist enabling optimized use of gene trap clones
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About
UniTrap UNI16210
Trapped Gene
Slc30a6 (
ENSMUSG00000024069
)
Vector Insertion
Chr 17: 74795009 - 74801251
Public Clones
(ggtc)
5SE223C04 (ggtc)
(ggtc)
3SE223C04 (ggtc)
(ggtc)
IST11013B8 (tigm)
IST14523C5 (tigm)
IST15056E4 (tigm)
Private Clones
OST465100 (lexicon)
OST450404 (lexicon)
OST170804 (lexicon)
OST34406 (lexicon)
Severity of mutation
(?)
Insertion after 0% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000692249 (Chr17:74794972..74795008 +)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
GCTGTACGGCTCCTTACCAT
Chr17:74794988..74795007
59.22
55
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000692249 (Chr17:74794972..74795008 +)
Downstram Exon
ENSMUSE00000383604 (Chr17:74801252..74801338 +)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
GCTGTACGGCTCCTTACCAT
Chr17:74794988..74795007
59.22
55
No primer for this exon
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000692249
Chr17:74794972..74795008
GCTGTACGGCTCCTTACCAT
Chr17:74794988..74795007
59.22
55
*** Putative Vector Insertion (Chr 17: 74795009 - 74801251) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000383604
Chr17:74801252..74801338
No primer for this exon
downstream
ENSMUSE00000394914
Chr17:74803344..74803428
TTGATTGCACCGAACAGAAG
Chr17:74803378..74803397
59.84
45
downstream
ENSMUSE00000369221
Chr17:74805025..74805067
No primer for this exon
downstream
ENSMUSE00000413190
Chr17:74806561..74806626
AGACAGGGCTAGGTTTCCTCA
Chr17:74806618..74806638
60.25
52.38
downstream
ENSMUSE00000367165
Chr17:74808186..74808266
CCCAATTGTGCCAAGACAGT
Chr17:74808245..74808264
60.95
50
downstream
ENSMUSE00000338492
Chr17:74808693..74808728
No primer for this exon
downstream
ENSMUSE00000375948
Chr17:74809674..74809768
GCATCGTGAACAGGTTGAAA
Chr17:74809732..74809751
59.7
45
downstream
ENSMUSE00000335967
Chr17:74811614..74811662
TCCTGAAGCCAGCTCGTACT
Chr17:74811641..74811660
60.16
55
downstream
ENSMUSE00000137945
Chr17:74811947..74812066
CTGGGATAAGGCCACACAAG
Chr17:74811969..74811988
60.51
55
downstream
ENSMUSE00000137950
Chr17:74814960..74815062
TACATGGTGCCAAACGTCAT
Chr17:74815028..74815047
59.85
45
downstream
ENSMUSE00000137948
Chr17:74817960..74818007
No primer for this exon
downstream
ENSMUSE00000137946
Chr17:74818872..74818940
CTAGCACCCCATCCAAGGTA
Chr17:74818899..74818918
59.95
55
downstream
ENSMUSE00000656979
Chr17:74822357..74823569
AAGGACCATTTGTTCGTTCG
Chr17:74822413..74822432
59.97
45
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
GCAGACATTCTCTCCCGTTC
Chr17:74798015..74798035
59.81
55
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
GCAGACATTCTCTCCCGTTC
Chr17:74798015..74798035
59.81
55
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000024069