Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI16298
Trapped Gene
Idh3g (ENSMUSG00000002010)
Vector Insertion
Chr X: 71025048 - 71025315
Public Clones not available
Private Clones OST463699 (lexicon)
Severity of mutation (?) Insertion after 78% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000208963 (ChrX:71025316..71025462 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000208963 (ChrX:71025316..71025462 -)
Downstram Exon
ENSMUSE00000208964 (ChrX:71024953..71025047 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000343243 ChrX:71032114..71032204 No primer for this exon
upstream ENSMUSE00000271758 ChrX:71028014..71028055 No primer for this exon
upstream ENSMUSE00000655218 ChrX:71027637..71027648 No primer for this exon
upstream ENSMUSE00000208970 ChrX:71027345..71027442 No primer for this exon
upstream ENSMUSE00000208952 ChrX:71027016..71027128 No primer for this exon
upstream ENSMUSE00000208965 ChrX:71026491..71026551 No primer for this exon
upstream ENSMUSE00000208954 ChrX:71026237..71026369 No primer for this exon
upstream ENSMUSE00000208967 ChrX:71025913..71026046 No primer for this exon
upstream ENSMUSE00000208969 ChrX:71025552..71025654 No primer for this exon
upstream ENSMUSE00000208963 ChrX:71025316..71025462 No primer for this exon

*** Putative Vector Insertion (Chr X: 71025048 - 71025315) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000208964 ChrX:71024953..71025047 No primer for this exon
downstream ENSMUSE00000208958 ChrX:71024805..71024865 No primer for this exon
downstream ENSMUSE00000384240 ChrX:71024306..71024510 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CTTGTGTAATCGCCTTGCAG ChrX:71025250..71025270 59.49 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CAGTGAGTAGCCAGGCACAA ChrX:71025296..71025316 60.05 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000002010