Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI16361
Trapped Gene
Ubl3 (ENSMUSG00000001687)
Vector Insertion
Chr 5: 149317764 - 149318021
Public Clones (sanger)
Private Clones OST462381 (lexicon) OST317629 (lexicon) OST315364 (lexicon)
Severity of mutation (?) Insertion after 86% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000191055 (Chr5:149318022..149318099 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000191055 (Chr5:149318022..149318099 -)
Downstram Exon
ENSMUSE00000647629 (Chr5:149316239..149317763 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000684215 Chr5:149363529..149364041 No primer for this exon
upstream ENSMUSE00000404861 Chr5:149323470..149323578 No primer for this exon
upstream ENSMUSE00000191057 Chr5:149320845..149320931 No primer for this exon
upstream ENSMUSE00000191055 Chr5:149318022..149318099 No primer for this exon

*** Putative Vector Insertion (Chr 5: 149317764 - 149318021) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000647629 Chr5:149316239..149317763 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ACCTCACTTGAGCCATCGAG Chr5:149317983..149318003 60.41 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ACCTCACTTGAGCCATCGAG Chr5:149317983..149318003 60.41 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 TGGCAAAACAACAGTGATGC Chr5:149318062..149318082 60.71 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 TGGCAAAACAACAGTGATGC Chr5:149318062..149318082 60.71 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000001687