Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI16429
Trapped Gene
Gpbp1 (ENSMUSG00000032745)
Vector Insertion
Chr 13: 112216139 - 112216860
Public Clones not available
Private Clones OST461482 (lexicon) OST385774 (lexicon) OST379927 (lexicon) OST358227 (lexicon)
OST337413 (lexicon) OST332802 (lexicon) OST325647 (lexicon) OST51866 (lexicon)
OST40075 (lexicon) OST38891 (lexicon) OST34865 (lexicon)
Severity of mutation (?) Insertion after 89% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000679704 (Chr13:112216369..112216859 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CTTCAAGTTTGGACCCTGGA Chr13:112216784..112216803 60.08 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000679704 (Chr13:112216369..112216859 -)
Downstram Exon
ENSMUSE00000610286 (Chr13:112216140..112216859 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CTTCAAGTTTGGACCCTGGA Chr13:112216784..112216803 60.08 50 CACTCTGCTCCGGTTTTAGC Chr13:112216158..112216177 60.01 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000120497 Chr13:112279926..112280047 GGCAAAAGGGGATTATTCAA Chr13:112280000..112280019 58.88 40
upstream ENSMUSE00000610285 Chr13:112279926..112280236 GGGACTGAGACTGGTTGTGG Chr13:112280033..112280052 60.56 60
upstream ENSMUSE00000316427 Chr13:112277631..112278578 TGGTTGAATTTGGCGTATGA Chr13:112278558..112278577 59.93 40
upstream ENSMUSE00000706092 Chr13:112277631..112278123 TCTTGTGCAGTCCTTTTGGA Chr13:112277635..112277654 59.42 45
upstream ENSMUSE00000316419 Chr13:112257059..112257178 CATGAGGTGTTGAAGCCTTG Chr13:112257151..112257170 59.29 50
upstream ENSMUSE00000709405 Chr13:112257059..112257178 CATGAGGTGTTGAAGCCTTG Chr13:112257151..112257170 59.29 50
upstream ENSMUSE00000316480 Chr13:112243581..112243704 TGGCTTTGATTCTGGTATTGG Chr13:112243595..112243615 59.95 42.86
upstream ENSMUSE00000441336 Chr13:112243232..112243455 AAATTCCCGTTCTCGTAGCA Chr13:112243345..112243364 59.71 45
upstream ENSMUSE00000316470 Chr13:112239141..112239207 ATCTTTAGCTGCGGGTGTTT Chr13:112239144..112239163 58.86 45
upstream ENSMUSE00000316408 Chr13:112238104..112238163 CTACACGCCCAGACACACAC Chr13:112238142..112238161 60.23 60
upstream ENSMUSE00000369274 Chr13:112230840..112231024 CTGGATTCCCAGTAGCAGGA Chr13:112230927..112230946 60.21 55
upstream ENSMUSE00000316459 Chr13:112229312..112229452 TTTTAAGTCAACGGCCAAGAA Chr13:112229343..112229363 59.74 38.1
upstream ENSMUSE00000316453 Chr13:112228165..112228332 ATGCGCAGCGATAAAAAGAG Chr13:112228241..112228260 60.5 45
upstream ENSMUSE00000679708 Chr13:112226605..112226792 GCAATGCCTCGATGATTTCT Chr13:112226676..112226695 60.19 45
upstream ENSMUSE00000568902 Chr13:112223756..112223858 TGCTCCCTTAACTGAGGATGA Chr13:112223787..112223807 59.82 47.62
upstream ENSMUSE00000679704 Chr13:112216369..112216859 CTTCAAGTTTGGACCCTGGA Chr13:112216784..112216803 60.08 50
upstream ENSMUSE00000610286 Chr13:112216140..112216859 GCTAAAACCGGAGCAGAGTG Chr13:112216180..112216199 60.01 55

*** Putative Vector Insertion (Chr 13: 112216139 - 112216860) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000568901 Chr13:112215890..112216859 CACTCTGCTCCGGTTTTAGC Chr13:112216158..112216177 60.01 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ATGGCCTGATAATCGCCTTG Chr13:112216798..112216818 62.22 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GAAAAATGGCCTGACGTGAC Chr13:112216803..112216823 60.5 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000032745