Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI16513
Trapped Gene
Pgs1 (ENSMUSG00000017715)
Vector Insertion
Chr 11: 117881047 - 117884718
Public Clones not available
Private Clones OST459464 (lexicon) OST259689 (lexicon) OST97468 (lexicon)
Severity of mutation (?) Insertion after 100% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000346452 (Chr11:117880921..117881046 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000346452 (Chr11:117880921..117881046 +)
Downstram Exon
ENSMUSE00000384302 (Chr11:117884719..117885325 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000427949 Chr11:117848171..117848327 No primer for this exon
upstream ENSMUSE00000367710 Chr11:117858755..117858944 No primer for this exon
upstream ENSMUSE00000151763 Chr11:117861513..117861590 No primer for this exon
upstream ENSMUSE00000669468 Chr11:117862336..117862347 No primer for this exon
upstream ENSMUSE00000151762 Chr11:117862930..117863029 No primer for this exon
upstream ENSMUSE00000151767 Chr11:117863665..117863854 No primer for this exon
upstream ENSMUSE00000151764 Chr11:117864655..117864833 No primer for this exon
upstream ENSMUSE00000360045 Chr11:117866684..117867205 No primer for this exon
upstream ENSMUSE00000398350 Chr11:117875888..117876036 No primer for this exon
upstream ENSMUSE00000669467 Chr11:117875961..117876036 No primer for this exon
upstream ENSMUSE00000346452 Chr11:117880921..117881046 No primer for this exon
upstream ENSMUSE00000669466 Chr11:117880921..117881046 No primer for this exon

*** Putative Vector Insertion (Chr 11: 117881047 - 117884718) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000384302 Chr11:117884719..117885325 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ACAGCATGTGGTGACAGCTC Chr11:117881022..117881042 59.9 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ACAGCATGTGGTGACAGCTC Chr11:117881022..117881042 59.9 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000017715