Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI16716
Trapped Gene
Rabgef1 (ENSMUSG00000025340)
Vector Insertion
Chr 5: 130663030 - 130663457
Public Clones not available
Private Clones OST455573 (lexicon) OST321837 (lexicon) OST298184 (lexicon) OST268908 (lexicon)
OST222140 (lexicon) OST185754 (lexicon) OST180277 (lexicon) OST171492 (lexicon)
OST167560 (lexicon) OST166422 (lexicon) OST147176 (lexicon) OST147174 (lexicon)
OST130010 (lexicon) OST128184 (lexicon) OST94722 (lexicon) OST59558 (lexicon)
OST57470 (lexicon) OST53090 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000714179 (Chr5:130663031..130663456 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CTGGAGGGAGGAGTACCACA Chr5:130663391..130663410 60.1 60 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000714179 (Chr5:130663031..130663456 +)
Downstram Exon
ENSMUSE00000711184 (Chr5:130663271..130663456 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CTGGAGGGAGGAGTACCACA Chr5:130663391..130663410 60.1 60 TTCGGACTTCAGGCTCATCT Chr5:130663298..130663317 59.95 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000712071 Chr5:130647668..130647764 AGGGAGCCGAGTGACTGAAG Chr5:130647700..130647719 61.91 60

*** Putative Vector Insertion (Chr 5: 130663030 - 130663457) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000714179 Chr5:130663031..130663456 TTCGGACTTCAGGCTCATCT Chr5:130663298..130663317 59.95 50
downstream ENSMUSE00000310054 Chr5:130663050..130663456 TTCGGACTTCAGGCTCATCT Chr5:130663298..130663317 59.95 50
downstream ENSMUSE00000711184 Chr5:130663271..130663456 TTCGGACTTCAGGCTCATCT Chr5:130663298..130663317 59.95 50
downstream ENSMUSE00000149942 Chr5:130666747..130666913 TGGTGACTTTTCGGGTTTTC Chr5:130666868..130666887 59.95 45
downstream ENSMUSE00000709554 Chr5:130673817..130673986 GTCCGTCTCAATGCTGGTTT Chr5:130673884..130673903 60.12 50
downstream ENSMUSE00000149947 Chr5:130673820..130673986 GTCCGTCTCAATGCTGGTTT Chr5:130673884..130673903 60.12 50
downstream ENSMUSE00000149944 Chr5:130682879..130682960 ACGGGTCTGCATTCTTTCAG Chr5:130682956..130682975 60.26 50
downstream ENSMUSE00000149946 Chr5:130684556..130684688 No primer for this exon
downstream ENSMUSE00000718174 Chr5:130684556..130684622 No primer for this exon
downstream ENSMUSE00000149948 Chr5:130686294..130686385 CATCTGAGGCGTTACCCAGT Chr5:130686324..130686343 60.13 55
downstream ENSMUSE00000149945 Chr5:130687732..130687988 GCCCTTCAGGACGATGTAGA Chr5:130687892..130687911 60.22 55
downstream ENSMUSE00000344215 Chr5:130688704..130690206 GGCCAACTCTCAGACTCCTG Chr5:130688828..130688847 59.99 60
downstream ENSMUSE00000709075 Chr5:130688704..130690212 GGCCAACTCTCAGACTCCTG Chr5:130688828..130688847 59.99 60

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGTTAATCGCCTTGCAGCAC Chr5:130663078..130663098 60.42 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector

Come back to gene ENSMUSG00000025340