Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI16723
Trapped Gene
A830080D01Rik (ENSMUSG00000044150)
Vector Insertion
Chr X: 155986170 - 155986248
Public Clones not available
Private Clones OST455476 (lexicon) OST436273 (lexicon) OST381471 (lexicon) OST376158 (lexicon)
OST369351 (lexicon) OST343062 (lexicon) OST328317 (lexicon) OST299951 (lexicon)
OST263315 (lexicon) OST216628 (lexicon) OST204630 (lexicon) OST195516 (lexicon)
OST126847 (lexicon) OST125050 (lexicon) OST64779 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000402605 (ChrX:155986171..155986247 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
AAAGTGGATCAGGCAGCTCA ChrX:155986176..155986195 60.94 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000402605 (ChrX:155986171..155986247 +)
Downstram Exon
ENSMUSE00000714689 (ChrX:155986171..155986247 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
AAAGTGGATCAGGCAGCTCA ChrX:155986176..155986195 60.94 50 GCATATGAGCTGCCTGATCC ChrX:155986203..155986222 60.74 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000440515 ChrX:155970600..155970767 GGCTCTGGTGACGACTTCTC ChrX:155970742..155970761 59.99 60

*** Putative Vector Insertion (Chr X: 155986170 - 155986248) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000402605 ChrX:155986171..155986247 GCATATGAGCTGCCTGATCC ChrX:155986203..155986222 60.74 55
downstream ENSMUSE00000714689 ChrX:155986171..155986247 GCATATGAGCTGCCTGATCC ChrX:155986203..155986222 60.74 55
downstream ENSMUSE00000343320 ChrX:155989683..155990306 GAGCCTCTTCTGGGTGACTG ChrX:155990273..155990292 59.99 60
downstream ENSMUSE00000372666 ChrX:155991176..155991826 GTCATTGCAAGGCTCCAAAT ChrX:155991668..155991687 60.08 45
downstream ENSMUSE00000410616 ChrX:155993407..155993582 TTTTGCCAATAGCAACCAAA ChrX:155993471..155993490 59.19 35
downstream ENSMUSE00000359817 ChrX:155995375..155995489 No primer for this exon
downstream ENSMUSE00000397778 ChrX:155996267..155996330 No primer for this exon
downstream ENSMUSE00000388572 ChrX:155997770..155997885 CGCTCTTTTCTCCTTCTCTCA ChrX:155997836..155997856 58.94 47.62
downstream ENSMUSE00000691768 ChrX:156004360..156004474 TTGAAATCCATCAGCACGAA ChrX:156004420..156004439 60.2 40
downstream ENSMUSE00000543676 ChrX:156005851..156005946 TCAATGGCATCTTTTCTCTGC ChrX:156005884..156005904 60.35 42.86
downstream ENSMUSE00000691767 ChrX:156005851..156005946 TCAATGGCATCTTTTCTCTGC ChrX:156005884..156005904 60.35 42.86
downstream ENSMUSE00000543675 ChrX:156012877..156013029 CAAACCGTAAGCGCTGGTAT ChrX:156012973..156012992 60.15 50
downstream ENSMUSE00000691766 ChrX:156012877..156013029 CAAACCGTAAGCGCTGGTAT ChrX:156012973..156012992 60.15 50
downstream ENSMUSE00000543674 ChrX:156030003..156031013 AACAAAGAGTGCCCCTCAAA ChrX:156030266..156030285 59.71 45
downstream ENSMUSE00000691765 ChrX:156030003..156031013 AACAAAGAGTGCCCCTCAAA ChrX:156030266..156030285 59.71 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TAAAGGATGGCACGGTCAAT ChrX:155986202..155986222 60.33 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AAGTGGATCAGGCAGCTCAT ChrX:155986178..155986198 59.83 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000044150