Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI17011
Trapped Gene
Napepld (ENSMUSG00000044968)
Vector Insertion
Chr 5: 21176422 - 21181272
Public Clones (sanger)
Private Clones OST450938 (lexicon) OST429065 (lexicon)
Severity of mutation (?) Insertion after 79% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000401164 (Chr5:21181273..21181919 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TCCCATCGGAGCTTATGAAC Chr5:21181277..21181296 60.04 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000401164 (Chr5:21181273..21181919 -)
Downstram Exon
ENSMUSE00000383623 (Chr5:21176307..21176421 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TCCCATCGGAGCTTATGAAC Chr5:21181277..21181296 60.04 50 GGGTCTGCATGCTGGTATTT Chr5:21176370..21176389 59.96 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000458392 Chr5:21207023..21207185 CTCGATATCTGCGTGGAACA Chr5:21207072..21207091 59.82 50
upstream ENSMUSE00000655629 Chr5:21206793..21207212 GCTGTTAGCCTGGATCTTCG Chr5:21207143..21207162 59.98 55
upstream ENSMUSE00000386128 Chr5:21189231..21189263 AGTATGAGGACAGCCAGTCTCC Chr5:21189239..21189260 59.77 54.54
upstream ENSMUSE00000336105 Chr5:21188974..21189228 ACATCTCCATCCCGAATGTG Chr5:21189028..21189047 60.75 50
upstream ENSMUSE00000359041 Chr5:21188974..21189283 GCGCCAAGCTATCAGTATCC Chr5:21189218..21189237 59.83 55
upstream ENSMUSE00000715725 Chr5:21188974..21189283 GCGCCAAGCTATCAGTATCC Chr5:21189218..21189237 59.83 55
upstream ENSMUSE00000401164 Chr5:21181273..21181919 TCCCATCGGAGCTTATGAAC Chr5:21181277..21181296 60.04 50

*** Putative Vector Insertion (Chr 5: 21176422 - 21181272) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000383623 Chr5:21176307..21176421 GGGTCTGCATGCTGGTATTT Chr5:21176370..21176389 59.96 50
downstream ENSMUSE00000552507 Chr5:21168798..21171180 CCATCCCTAGACTCCCACAA Chr5:21170866..21170885 59.92 55
downstream ENSMUSE00000702080 Chr5:21168719..21171180 CCATCCCTAGACTCCCACAA Chr5:21170866..21170885 59.92 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CCAACTCTCGGCTTGTTTTT Chr5:21181244..21181264 59.35 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CCAACTCTCGGCTTGTTTTT Chr5:21181244..21181264 59.35 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 CGAGTCCTTTAGAGGCAGGAT Chr5:21181916..21181937 59.86 52.38 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 ATGCTGCTGTTTGCCTTTCT Chr5:21181932..21181952 60.02 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000044968