Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI1703
Trapped Gene
Diap2 (ENSMUSG00000034480)
Vector Insertion
Chr X: 126360484 - 126437435
Public Clones CF0683 (sanger) AM0811 (sanger) RRJ302 (baygenomics) RRP123 (baygenomics)
RRM097 (baygenomics)
Private Clones not available
Severity of mutation (?) Insertion after 29% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000695192 (ChrX:126360463..126360483 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000695192 (ChrX:126360463..126360483 +)
Downstram Exon
ENSMUSE00000546626 (ChrX:126437436..126437575 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon TGACAGGATGGCTGGTTAAA ChrX:126437577..126437596 59.12 45

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000546634 ChrX:126284282..126284652 TTTGCCCTTGAGGAGACTTG ChrX:126284456..126284475 60.37 50
upstream ENSMUSE00000622646 ChrX:126284332..126284652 TTTGCCCTTGAGGAGACTTG ChrX:126284456..126284475 60.37 50
upstream ENSMUSE00000653827 ChrX:126284512..126284652 CCAACGAAGAGGAGACGAGA ChrX:126284618..126284637 60.52 55
upstream ENSMUSE00000695158 ChrX:126335931..126335963 No primer for this exon
upstream ENSMUSE00000546633 ChrX:126338549..126338725 TTCAGAAAATCTGCCACCAA ChrX:126338567..126338586 59.25 40
upstream ENSMUSE00000546630 ChrX:126355775..126355879 TAGCATCAAGCGTGAGATGG ChrX:126355828..126355847 59.97 50
upstream ENSMUSE00000695192 ChrX:126360463..126360483 No primer for this exon

*** Putative Vector Insertion (Chr X: 126360484 - 126437435) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000546626 ChrX:126437436..126437575 TGACAGGATGGCTGGTTAAA ChrX:126437577..126437596 59.12 45
downstream ENSMUSE00000546625 ChrX:126473781..126473855 GCTTCTCCAGCACATCCAGT ChrX:126473842..126473861 60.42 55
downstream ENSMUSE00000546623 ChrX:126473939..126474008 No primer for this exon
downstream ENSMUSE00000546621 ChrX:126477435..126477571 GTTGTTTCGGGTCAATTGCT ChrX:126477510..126477529 59.98 45
downstream ENSMUSE00000546617 ChrX:126478379..126478487 TGATGCCCCCTAAAAGTTTG ChrX:126478407..126478426 59.93 45
downstream ENSMUSE00000546615 ChrX:126487209..126487319 TGGAGAGGTGACAAGTGCAT ChrX:126487253..126487272 59.26 50
downstream ENSMUSE00000546613 ChrX:126490430..126490548 TGAGACGGTGGGATAATTCA ChrX:126490529..126490548 58.92 45
downstream ENSMUSE00000546602 ChrX:126491779..126491895 GTAAGGTTCCGCAGCAGTGT ChrX:126491845..126491864 60.32 55
downstream ENSMUSE00000546600 ChrX:126494580..126494698 GCTGCCGGTACTTGAAGTCT ChrX:126494671..126494690 59.5 55
downstream ENSMUSE00000546598 ChrX:126497456..126497520 AATTCCATTGCTTTTTGCTCA ChrX:126497512..126497532 59.71 33.33
downstream ENSMUSE00000546593 ChrX:126501062..126501166 CTCGAAGCTGCTGGATTTCT ChrX:126501164..126501183 59.72 50
downstream ENSMUSE00000546591 ChrX:126505815..126506129 AGACAAAGGTGGTGGAGGTG ChrX:126505997..126506016 60 55
downstream ENSMUSE00000546588 ChrX:126507031..126507145 AATGTCAAGGCCAGTTTTGC ChrX:126507131..126507150 60.12 45
downstream ENSMUSE00000496232 ChrX:126581861..126581962 CTTTGGAGGCCCACTCCTAT ChrX:126581913..126581932 60.46 55
downstream ENSMUSE00000498913 ChrX:126584903..126584997 TGCGGTAAGATCCAAGGAAG ChrX:126584929..126584948 60.21 50
downstream ENSMUSE00000419196 ChrX:126608358..126608459 CTGGGCTAGTTCTCGGAGTG ChrX:126608411..126608430 60.01 60
downstream ENSMUSE00000350456 ChrX:126623063..126623302 CGTGAGACGAGGACGTAACA ChrX:126623101..126623120 59.9 55
downstream ENSMUSE00000207467 ChrX:126643788..126643917 TGCAAAAGGGTGGATTTTTG ChrX:126643834..126643853 60.84 40
downstream ENSMUSE00000419154 ChrX:126713472..126713596 TTATCATGGTGCGATTCTGC ChrX:126713580..126713599 59.65 45
downstream ENSMUSE00000371850 ChrX:126793613..126793777 TGTGTTCGGGTCAAAAATGA ChrX:126793726..126793745 59.94 40
downstream ENSMUSE00000207471 ChrX:126824390..126824525 TTTGCAAGCTTTGCTCTCCT ChrX:126824460..126824479 60.27 45
downstream ENSMUSE00000346672 ChrX:126862502..126862597 GCACCTGACTGTAGGGCTTC ChrX:126862559..126862578 59.87 60
downstream ENSMUSE00000622643 ChrX:126862502..126863393 GCACCTGACTGTAGGGCTTC ChrX:126862559..126862578 59.87 60
downstream ENSMUSE00000653826 ChrX:126862502..126862704 GCACCTGACTGTAGGGCTTC ChrX:126862559..126862578 59.87 60
downstream ENSMUSE00000546574 ChrX:126995407..126995471 CGAGAGCGAGATCTTTCCAG ChrX:126995446..126995465 60.23 55
downstream ENSMUSE00000695156 ChrX:126995407..127000370 GGCCTCTGTTGTAGCCTCTG ChrX:126998087..126998106 60.01 60

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ATGTCACGGAGAAGGCGTTA ChrX:126420472..126420492 60.66 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TTTCCGTGACTGGGAAAACC ChrX:126420531..126420551 62.17 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000034480