Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI17082
Trapped Gene
Helz (ENSMUSG00000020721)
Vector Insertion
Chr 11: 107416890 - 107436251
Public Clones not available
Private Clones OST449581 (lexicon) OST389682 (lexicon) OST316961 (lexicon) OST307827 (lexicon)
OST284226 (lexicon) OST277371 (lexicon) OST276233 (lexicon) OST219026 (lexicon)
OST217293 (lexicon) OST166762 (lexicon) OST68373 (lexicon) OST63597 (lexicon)
OST59643 (lexicon) OST45436 (lexicon) OST45411 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000646744 (Chr11:107416833..107416889 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000646744 (Chr11:107416833..107416889 +)
Downstram Exon
ENSMUSE00000717635 (Chr11:107436252..107436479 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000646745 Chr11:107409270..107409295 No primer for this exon
upstream ENSMUSE00000483326 Chr11:107409274..107409295 No primer for this exon
upstream ENSMUSE00000670998 Chr11:107409282..107409295 No primer for this exon
upstream ENSMUSE00000451387 Chr11:107410660..107410715 No primer for this exon
upstream ENSMUSE00000646744 Chr11:107416833..107416889 No primer for this exon

*** Putative Vector Insertion (Chr 11: 107416890 - 107436251) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000485768 Chr11:107436252..107436479 No primer for this exon
downstream ENSMUSE00000717635 Chr11:107436252..107436479 No primer for this exon
downstream ENSMUSE00000490303 Chr11:107439177..107439213 No primer for this exon
downstream ENSMUSE00000476268 Chr11:107454020..107454144 No primer for this exon
downstream ENSMUSE00000488696 Chr11:107456453..107456509 No primer for this exon
downstream ENSMUSE00000489640 Chr11:107460464..107460515 No primer for this exon
downstream ENSMUSE00000464405 Chr11:107461590..107461665 No primer for this exon
downstream ENSMUSE00000465277 Chr11:107463624..107463822 No primer for this exon
downstream ENSMUSE00000463402 Chr11:107464250..107464357 No primer for this exon
downstream ENSMUSE00000515186 Chr11:107465380..107465677 No primer for this exon
downstream ENSMUSE00000466985 Chr11:107475165..107475432 No primer for this exon
downstream ENSMUSE00000473629 Chr11:107480297..107480630 No primer for this exon
downstream ENSMUSE00000468969 Chr11:107481398..107481528 No primer for this exon
downstream ENSMUSE00000460723 Chr11:107487849..107488028 No primer for this exon
downstream ENSMUSE00000474525 Chr11:107488664..107488768 No primer for this exon
downstream ENSMUSE00000646736 Chr11:107488667..107488768 No primer for this exon
downstream ENSMUSE00000499887 Chr11:107493486..107493664 No primer for this exon
downstream ENSMUSE00000346033 Chr11:107496294..107496412 No primer for this exon
downstream ENSMUSE00000396126 Chr11:107497480..107497625 No primer for this exon
downstream ENSMUSE00000352961 Chr11:107499094..107499241 No primer for this exon
downstream ENSMUSE00000347637 Chr11:107507364..107507547 No primer for this exon
downstream ENSMUSE00000387812 Chr11:107510426..107510654 No primer for this exon
downstream ENSMUSE00000344638 Chr11:107517358..107517562 No primer for this exon
downstream ENSMUSE00000108659 Chr11:107522409..107522460 No primer for this exon
downstream ENSMUSE00000108690 Chr11:107523156..107523346 No primer for this exon
downstream ENSMUSE00000108662 Chr11:107524922..107525129 No primer for this exon
downstream ENSMUSE00000108658 Chr11:107527313..107527392 No primer for this exon
downstream ENSMUSE00000322856 Chr11:107531349..107531985 No primer for this exon
downstream ENSMUSE00000513753 Chr11:107532361..107532595 No primer for this exon
downstream ENSMUSE00000646728 Chr11:107532361..107533601 No primer for this exon
downstream ENSMUSE00000401493 Chr11:107533843..107534359 No primer for this exon
downstream ENSMUSE00000108661 Chr11:107546605..107546857 No primer for this exon
downstream ENSMUSE00000502524 Chr11:107547699..107548257 No primer for this exon
downstream ENSMUSE00000670997 Chr11:107547699..107555140 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CATAGCCCTGGGAGTCAAAC Chr11:107434846..107434866 59.55 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CATAGCCCTGGGAGTCAAAC Chr11:107434846..107434866 59.55 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000020721