Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI17313
Trapped Gene
Lrrc46 (ENSMUSG00000020878)
Vector Insertion
Chr 11: 96900195 - 96902180
Public Clones not available
Private Clones OST445271 (lexicon) OST231069 (lexicon)
Severity of mutation (?) Insertion after 13% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000111046 (Chr11:96902181..96902286 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000111046 (Chr11:96902181..96902286 -)
Downstram Exon
ENSMUSE00000111048 (Chr11:96900086..96900194 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000289096 Chr11:96902397..96902658 No primer for this exon
upstream ENSMUSE00000111046 Chr11:96902181..96902286 No primer for this exon

*** Putative Vector Insertion (Chr 11: 96900195 - 96902180) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000111048 Chr11:96900086..96900194 No primer for this exon
downstream ENSMUSE00000111045 Chr11:96897784..96897830 No primer for this exon
downstream ENSMUSE00000111044 Chr11:96897410..96897519 No primer for this exon
downstream ENSMUSE00000111051 Chr11:96897186..96897255 No primer for this exon
downstream ENSMUSE00000111047 Chr11:96896773..96896918 No primer for this exon
downstream ENSMUSE00000368394 Chr11:96895912..96896338 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TAGAAGGCGGGAACAGTGAC Chr11:96902132..96902152 60.26 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TAGAAGGCGGGAACAGTGAC Chr11:96902132..96902152 60.26 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000020878