Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI17333
Trapped Gene
Mt2 (ENSMUSG00000031762)
Vector Insertion
Chr 8: 96696776 - 96697026
Public Clones not available
Private Clones OST444955 (lexicon) OST444953 (lexicon) OST179541 (lexicon)
Severity of mutation (?) Insertion after 16% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000331875 (Chr8:96696684..96696775 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CCGATCTCTCGTCGATCTTC Chr8:96696708..96696727 59.91 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000331875 (Chr8:96696684..96696775 +)
Downstram Exon
ENSMUSE00000212752 (Chr8:96697027..96697092 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CCGATCTCTCGTCGATCTTC Chr8:96696708..96696727 59.91 55 No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000331875 Chr8:96696684..96696775 CCGATCTCTCGTCGATCTTC Chr8:96696708..96696727 59.91 55

*** Putative Vector Insertion (Chr 8: 96696776 - 96697026) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000212752 Chr8:96697027..96697092 No primer for this exon
downstream ENSMUSE00000212749 Chr8:96697236..96697462 CACTTGTCGGAAGCCTCTTT Chr8:96697314..96697333 59.47 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGCTTTTTGTAATCGCCTTG Chr8:96696818..96696838 59.72 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GACAGGCTTTTTGCGTGACT Chr8:96696814..96696834 60.44 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000031762