Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI17335
Trapped Gene
Mrpl24 (ENSMUSG00000019710)
Vector Insertion
Chr 3: 87725738 - 87725971
Public Clones not available
Private Clones OST444843 (lexicon) OST433655 (lexicon) OST402760 (lexicon) OST379492 (lexicon)
OST311518 (lexicon) OST310954 (lexicon) OST310950 (lexicon) OST309103 (lexicon)
OST245044 (lexicon) OST198763 (lexicon) OST81189 (lexicon) OST72572 (lexicon)
OST68360 (lexicon) OST46210 (lexicon) OST34535 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000175792 (Chr3:87725739..87725970 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000175792 (Chr3:87725739..87725970 +)
Downstram Exon
ENSMUSE00000712426 (Chr3:87725739..87725970 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000720096 Chr3:87723428..87723750 No primer for this exon
upstream ENSMUSE00000175795 Chr3:87723466..87723627 No primer for this exon
upstream ENSMUSE00000708200 Chr3:87723485..87723573 No primer for this exon
upstream ENSMUSE00000719407 Chr3:87725531..87725970 No primer for this exon

*** Putative Vector Insertion (Chr 3: 87725738 - 87725971) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000175792 Chr3:87725739..87725970 No primer for this exon
downstream ENSMUSE00000712426 Chr3:87725739..87725970 No primer for this exon
downstream ENSMUSE00000175794 Chr3:87726053..87726148 No primer for this exon
downstream ENSMUSE00000175793 Chr3:87726310..87726413 No primer for this exon
downstream ENSMUSE00000175797 Chr3:87726857..87726987 No primer for this exon
downstream ENSMUSE00000175796 Chr3:87727132..87727355 No primer for this exon
downstream ENSMUSE00000717961 Chr3:87727132..87727584 No primer for this exon
downstream ENSMUSE00000721275 Chr3:87727132..87727594 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
  No PCR available

Come back to gene ENSMUSG00000019710