Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI17392
Trapped Gene
Dnajc5 (ENSMUSG00000000826)
Vector Insertion
Chr 2: 181281075 - 181281194
Public Clones not available
Private Clones OST443568 (lexicon) OST434576 (lexicon) OST420634 (lexicon) OST377635 (lexicon)
OST333995 (lexicon) OST302884 (lexicon) OST274854 (lexicon) OST206467 (lexicon)
OST161487 (lexicon) OST126586 (lexicon) OST95921 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000170834 (Chr2:181281076..181281193 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000170834 (Chr2:181281076..181281193 +)
Downstram Exon
ENSMUSE00000712592 (Chr2:181281076..181281193 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000509198 Chr2:181255210..181255402 No primer for this exon
upstream ENSMUSE00000706029 Chr2:181255231..181255402 No primer for this exon
upstream ENSMUSE00000678050 Chr2:181255256..181255402 No primer for this exon
upstream ENSMUSE00000678047 Chr2:181255652..181255763 No primer for this exon
upstream ENSMUSE00000678049 Chr2:181255940..181255992 No primer for this exon

*** Putative Vector Insertion (Chr 2: 181281075 - 181281194) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000170834 Chr2:181281076..181281193 No primer for this exon
downstream ENSMUSE00000712592 Chr2:181281076..181281193 No primer for this exon
downstream ENSMUSE00000170833 Chr2:181282045..181282258 No primer for this exon
downstream ENSMUSE00000170835 Chr2:181283375..181283546 No primer for this exon
downstream ENSMUSE00000706028 Chr2:181283631..181283705 No primer for this exon
downstream ENSMUSE00000545485 Chr2:181283978..181286932 No primer for this exon
downstream ENSMUSE00000678046 Chr2:181283978..181284295 No primer for this exon
downstream ENSMUSE00000678048 Chr2:181283978..181286937 No primer for this exon
downstream ENSMUSE00000678052 Chr2:181283978..181284077 No primer for this exon
downstream ENSMUSE00000706027 Chr2:181283978..181286938 No primer for this exon
downstream ENSMUSE00000678051 Chr2:181285618..181285636 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
  No PCR available

Come back to gene ENSMUSG00000000826