Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI17405
Trapped Gene
Dusp12 (ENSMUSG00000026659)
Vector Insertion
Chr 1: 172811160 - 172815197
Public Clones (sanger) E098C09 (ggtc) E089F06 (ggtc) E098C09 (ggtc) CMHD-GT_469A6-3 (cmhd)
IST12628D7 (tigm) IST14514C11 (tigm) IST14918H8 (tigm) IST14250G7 (tigm)
IST10106C12 (tigm) IST14298G2 (tigm) IST10106A12 (tigm) IST10912A1 (tigm)
IST14614D9 (tigm) IST10106D11 (tigm) IST10885F11 (tigm) IST10106A11 (tigm)
IST10384F4 (tigm)
Private Clones OST443391 (lexicon) OST125914 (lexicon)
Severity of mutation (?) Insertion after 33% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000495810 (Chr1:172815198..172815552 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CTGACGGTGGACTCTGAACC Chr1:172815351..172815370 60.71 60 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000495810 (Chr1:172815198..172815552 -)
Downstram Exon
ENSMUSE00000161686 (Chr1:172811046..172811159 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CTGACGGTGGACTCTGAACC Chr1:172815351..172815370 60.71 60 ACAGCAACACTGCGACTGAC Chr1:172811108..172811127 60.1 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000495810 Chr1:172815198..172815552 CTGACGGTGGACTCTGAACC Chr1:172815351..172815370 60.71 60

*** Putative Vector Insertion (Chr 1: 172811160 - 172815197) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000161686 Chr1:172811046..172811159 ACAGCAACACTGCGACTGAC Chr1:172811108..172811127 60.1 55
downstream ENSMUSE00000161687 Chr1:172810704..172810822 AAAAGGCACTGGACGTATCG Chr1:172810724..172810743 60.13 50
downstream ENSMUSE00000474519 Chr1:172810261..172810357 GGGTCAACAGCGAAGAGTTC Chr1:172810296..172810315 59.85 55
downstream ENSMUSE00000515104 Chr1:172810261..172810357 GGGTCAACAGCGAAGAGTTC Chr1:172810296..172810315 59.85 55
downstream ENSMUSE00000506000 Chr1:172809791..172809977 ATCCATAACGCCCAACAGAG Chr1:172809775..172809794 59.96 50
downstream ENSMUSE00000514238 Chr1:172809791..172809977 ATCCATAACGCCCAACAGAG Chr1:172809775..172809794 59.96 50
downstream ENSMUSE00000435073 Chr1:172804320..172804701 GGGTTATCCATCGACCACAG Chr1:172804598..172804617 60.19 55
downstream ENSMUSE00000593058 Chr1:172804320..172804701 GGGTTATCCATCGACCACAG Chr1:172804598..172804617 60.19 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ATAATCGCCTTGCAGCACAT Chr1:172812127..172812147 60.64 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TCTCCGTGACTGGGAAAACC Chr1:172815130..172815150 62.38 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 ATAATCGCCTTGCAGCACAT Chr1:172812483..172812503 60.64 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 GTCACTCTAGCCGGTGACTTG Chr1:172815574..172815595 59.92 57.14 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000026659