Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI17496
Trapped Gene
Rnf185 (ENSMUSG00000020448)
Vector Insertion
Chr 11: 3332402 - 3332624
Public Clones not available
Private Clones OST441716 (lexicon) OST422304 (lexicon) OST352110 (lexicon) OST268411 (lexicon)
OST222726 (lexicon) OST221031 (lexicon) OST205458 (lexicon) OST201384 (lexicon)
OST45670 (lexicon) OST32845 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000581549 (Chr11:3332403..3332623 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000581549 (Chr11:3332403..3332623 -)
Downstram Exon
ENSMUSE00000581554 (Chr11:3332403..3332623 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000416051 Chr11:3352212..3352309 No primer for this exon
upstream ENSMUSE00000595379 Chr11:3352212..3352366 No primer for this exon
upstream ENSMUSE00000416041 Chr11:3338208..3338326 No primer for this exon
upstream ENSMUSE00000682440 Chr11:3338208..3338270 No primer for this exon
upstream ENSMUSE00000581549 Chr11:3332403..3332623 No primer for this exon
upstream ENSMUSE00000581554 Chr11:3332403..3332623 No primer for this exon

*** Putative Vector Insertion (Chr 11: 3332402 - 3332624) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000656344 Chr11:3328887..3328905 No primer for this exon
downstream ENSMUSE00000104745 Chr11:3326569..3326681 No primer for this exon
downstream ENSMUSE00000104747 Chr11:3325285..3325339 No primer for this exon
downstream ENSMUSE00000104743 Chr11:3319865..3319982 No primer for this exon
downstream ENSMUSE00000332999 Chr11:3315986..3318090 No primer for this exon
downstream ENSMUSE00000706248 Chr11:3315985..3318090 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
  No PCR available

Come back to gene ENSMUSG00000020448