Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI17556
Trapped Gene
Acp5 (ENSMUSG00000001348)
Vector Insertion
Chr 9: 21934574 - 21934966
Public Clones not available
Private Clones OST440764 (lexicon) OST407811 (lexicon) OST125728 (lexicon) OST98333 (lexicon)
OST16115 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000702441 (Chr9:21934967..21935098 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000702441 (Chr9:21934967..21935098 -)
Downstram Exon
ENSMUSE00000217906 (Chr9:21934307..21934573 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000702450 Chr9:21936181..21936259 No primer for this exon
upstream ENSMUSE00000702441 Chr9:21934967..21935098 No primer for this exon

*** Putative Vector Insertion (Chr 9: 21934574 - 21934966) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000217906 Chr9:21934307..21934573 No primer for this exon
downstream ENSMUSE00000217900 Chr9:21933975..21934102 No primer for this exon
downstream ENSMUSE00000217902 Chr9:21932119..21932464 No primer for this exon
downstream ENSMUSE00000702440 Chr9:21931190..21931706 No primer for this exon
downstream ENSMUSE00000638189 Chr9:21931175..21931706 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CAGCCTCTCTGACCACCTGT Chr9:21934977..21934997 60.46 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CAGCCTCTCTGACCACCTGT Chr9:21934977..21934997 60.46 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000001348