Tools for the biologist enabling optimized use of gene trap clones
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About
UniTrap UNI17702
Trapped Gene
1700061G19Rik (
ENSMUSG00000024209
)
Vector Insertion
Chr 17: 57024647 - 57025863
Public Clones
IST12685G12 (tigm)
IST10904F3 (tigm)
IST13124A7 (tigm)
IST11996H11 (tigm)
IST12685G12 (tigm)
IST14857G12 (tigm)
Private Clones
OST437793 (lexicon)
OST205118 (lexicon)
OST180331 (lexicon)
Severity of mutation
(?)
Insertion after 84% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000139761 (Chr17:57024507..57024646 +)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
GGGAAATGGTCAACCCGTAT
Chr17:57024528..57024547
60.81
50
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000139761 (Chr17:57024507..57024646 +)
Downstram Exon
ENSMUSE00000283440 (Chr17:57025864..57026110 +)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
GGGAAATGGTCAACCCGTAT
Chr17:57024528..57024547
60.81
50
AGGTTACTCCGAGCCTCTCC
Chr17:57025907..57025926
59.84
60
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000284015
Chr17:57014906..57015103
TGATAAAGTGGCAGCCTGTG
Chr17:57015009..57015028
59.86
50
upstream
ENSMUSE00000283990
Chr17:57015511..57015819
CTGTGGACACCTGGTCCTCT
Chr17:57015611..57015630
60.15
60
upstream
ENSMUSE00000283969
Chr17:57016786..57017012
GCAACTGCTCACCTACATCG
Chr17:57016949..57016968
59.47
55
upstream
ENSMUSE00000376498
Chr17:57019874..57019962
GTCTGGAACGCTTTCATGGT
Chr17:57019878..57019897
60.12
50
upstream
ENSMUSE00000283885
Chr17:57020369..57020489
TCGCTGAGACCTCAGAAATG
Chr17:57020422..57020441
59.11
50
upstream
ENSMUSE00000493939
Chr17:57021590..57021670
TCCAGGGCTACTTGAAGCAT
Chr17:57021591..57021610
59.84
50
upstream
ENSMUSE00000283830
Chr17:57021964..57022113
ACTGAAGCCCAATCAGTGCT
Chr17:57022029..57022048
59.87
50
upstream
ENSMUSE00000139757
Chr17:57022203..57022409
GCTACCTGCCACTTTGCTTC
Chr17:57022279..57022298
60.02
55
upstream
ENSMUSE00000139751
Chr17:57022739..57022920
TCCACACCAACAAGAAGCTG
Chr17:57022893..57022912
59.87
50
upstream
ENSMUSE00000283755
Chr17:57023050..57023283
GTTGTACGGCTTGTCGGAGT
Chr17:57023215..57023234
60.18
55
upstream
ENSMUSE00000139749
Chr17:57024174..57024391
GGGCTTCTTGGATTTTGACA
Chr17:57024342..57024361
60.05
45
upstream
ENSMUSE00000139761
Chr17:57024507..57024646
GGGAAATGGTCAACCCGTAT
Chr17:57024528..57024547
60.81
50
*** Putative Vector Insertion (Chr 17: 57024647 - 57025863) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000283440
Chr17:57025864..57026110
AGGTTACTCCGAGCCTCTCC
Chr17:57025907..57025926
59.84
60
downstream
ENSMUSE00000693744
Chr17:57028060..57028327
GGCGCTTGAACGAAGATTAG
Chr17:57028182..57028201
59.98
50
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
GAGTAATCGCCTTGCAGCAC
Chr17:57024695..57024715
60.94
55
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
CACGGAGCAAGACTCTGACC
Chr17:57024653..57024673
61.01
60
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000024209