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About
UniTrap UNI1779
Trapped Gene
Slc10a7 (
ENSMUSG00000031684
)
Vector Insertion
Chr 8: 81186885 - 81186922
Public Clones
CE0352 (sanger)
Private Clones
not available
Severity of mutation
(?)
Insertion after 43% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000606945 (Chr8:81186886..81186921 +)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
ATTGTTGTGACTCCGGTGCT
Chr8:81186889..81186908
60.58
50
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000606945 (Chr8:81186886..81186921 +)
Downstram Exon
ENSMUSE00000249083 (Chr8:81186886..81186921 +)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
ATTGTTGTGACTCCGGTGCT
Chr8:81186889..81186908
60.58
50
AGCACCGGAGTCACAACAAT
Chr8:81186911..81186930
60.58
50
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000404952
Chr8:81033267..81033573
AATGGTTCATGGTCGGGATA
Chr8:81033502..81033521
60.01
45
upstream
ENSMUSE00000445432
Chr8:81033361..81033573
AATGGTTCATGGTCGGGATA
Chr8:81033502..81033521
60.01
45
upstream
ENSMUSE00000211734
Chr8:81039485..81039567
CGTCGCAACGATATTCTTCA
Chr8:81039522..81039541
59.83
45
upstream
ENSMUSE00000581693
Chr8:81049061..81049197
ATCCATCAACGAGTGGCTTT
Chr8:81049171..81049190
59.56
45
upstream
ENSMUSE00000581696
Chr8:81049061..81049197
ATCCATCAACGAGTGGCTTT
Chr8:81049171..81049190
59.56
45
upstream
ENSMUSE00000249095
Chr8:81054982..81055057
AAGGCAGTTGGTGGAAATGA
Chr8:81055037..81055056
60.49
45
upstream
ENSMUSE00000581692
Chr8:81054982..81055057
AAGGCAGTTGGTGGAAATGA
Chr8:81055037..81055056
60.49
45
upstream
ENSMUSE00000249088
Chr8:81102618..81102656
No primer for this exon
upstream
ENSMUSE00000606946
Chr8:81102618..81102656
No primer for this exon
*** Putative Vector Insertion (Chr 8: 81186885 - 81186922) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000249083
Chr8:81186886..81186921
AGCACCGGAGTCACAACAAT
Chr8:81186911..81186930
60.58
50
downstream
ENSMUSE00000606945
Chr8:81186886..81186921
AGCACCGGAGTCACAACAAT
Chr8:81186911..81186930
60.58
50
downstream
ENSMUSE00000606944
Chr8:81210484..81210567
GGAACCACCACCGTCATAAA
Chr8:81210554..81210573
60.62
50
downstream
ENSMUSE00000635949
Chr8:81210484..81210567
GGAACCACCACCGTCATAAA
Chr8:81210554..81210573
60.62
50
downstream
ENSMUSE00000211723
Chr8:81221065..81221230
GCTACTGCTGACCACACCAA
Chr8:81221136..81221155
59.9
55
downstream
ENSMUSE00000606943
Chr8:81221065..81221230
GCTACTGCTGACCACACCAA
Chr8:81221136..81221155
59.9
55
downstream
ENSMUSE00000211729
Chr8:81222419..81222470
AAGCTCAGCTGAACGGAGAC
Chr8:81222443..81222462
59.75
55
downstream
ENSMUSE00000682022
Chr8:81222419..81222470
AAGCTCAGCTGAACGGAGAC
Chr8:81222443..81222462
59.75
55
downstream
ENSMUSE00000211727
Chr8:81230774..81230847
TGGTGTGAACCCCGAGTTAT
Chr8:81230798..81230817
60.23
50
downstream
ENSMUSE00000682021
Chr8:81230774..81230847
TGGTGTGAACCCCGAGTTAT
Chr8:81230798..81230817
60.23
50
downstream
ENSMUSE00000211728
Chr8:81253517..81253662
CTCATGGCCTGCAAACACTA
Chr8:81253557..81253576
59.86
50
downstream
ENSMUSE00000606942
Chr8:81253517..81253662
CTCATGGCCTGCAAACACTA
Chr8:81253557..81253576
59.86
50
downstream
ENSMUSE00000341040
Chr8:81255508..81256050
CTGCACAAAGGCAGTACGAA
Chr8:81255848..81255867
60.05
50
downstream
ENSMUSE00000581694
Chr8:81255508..81257902
GAGGTACCTGACGCTTCTCG
Chr8:81256058..81256077
60.01
60
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
TCAGGGCATTGTTGTGACTC
Chr8:81186883..81186903
59.68
50
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
TCAGGGCATTGTTGTGACTC
Chr8:81186883..81186903
59.68
50
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000031684