Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI17866
Trapped Gene
Mcm7 (ENSMUSG00000029730)
Vector Insertion
Chr 5: 138606149 - 138606279
Public Clones not available
Private Clones OST434638 (lexicon) OST382252 (lexicon) OST340758 (lexicon) OST253183 (lexicon)
Severity of mutation (?) Insertion after 91% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000192153 (Chr5:138606280..138606389 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GCTCGACTTCGGATGGTAGA Chr5:138606370..138606389 60.36 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000192153 (Chr5:138606280..138606389 -)
Downstram Exon
ENSMUSE00000535291 (Chr5:138605817..138606148 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GCTCGACTTCGGATGGTAGA Chr5:138606370..138606389 60.36 55 TGAAGCCACGAGATATGCAG Chr5:138606017..138606036 59.97 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000686007 Chr5:138612954..138613090 GATGGCGCTTAAGGACTACG Chr5:138612966..138612985 59.87 55
upstream ENSMUSE00000304321 Chr5:138611545..138611624 No primer for this exon
upstream ENSMUSE00000192143 Chr5:138610788..138610952 GCCAAGCGCTACTCAAGACT Chr5:138610837..138610856 59.79 55
upstream ENSMUSE00000192142 Chr5:138610577..138610701 CCCCCAAAACCAGTATCCTT Chr5:138610596..138610615 60.05 50
upstream ENSMUSE00000192155 Chr5:138610326..138610506 CGGGAAGTACGAGCTGACTC Chr5:138610441..138610460 60.01 60
upstream ENSMUSE00000304275 Chr5:138610038..138610175 ACAGACTCGTGGCTCCAAAT Chr5:138610073..138610092 59.73 50
upstream ENSMUSE00000192141 Chr5:138609561..138609710 AGCGTCACTGGCATCTTCTT Chr5:138609598..138609617 60.02 50
upstream ENSMUSE00000192152 Chr5:138609286..138609400 GCCCACTGGATTGTGAAGAT Chr5:138609354..138609373 59.93 50
upstream ENSMUSE00000192150 Chr5:138609069..138609200 GTGGTGTGGACCAGTCTCCT Chr5:138609088..138609107 60.01 60
upstream ENSMUSE00000192151 Chr5:138608779..138608862 GTGTGGCCAAATCTCAGCTC Chr5:138608810..138608829 60.81 55
upstream ENSMUSE00000192145 Chr5:138608274..138608667 GGATTCTCACCACGCTCAAT Chr5:138608432..138608451 60.08 50
upstream ENSMUSE00000192147 Chr5:138607911..138607994 TCACCTATGTCCACCAGCAC Chr5:138607961..138607980 59.55 55
upstream ENSMUSE00000192148 Chr5:138607138..138607306 TTCTCCGACTTTCCACTGCT Chr5:138607141..138607160 59.99 50
upstream ENSMUSE00000192153 Chr5:138606280..138606389 GCTCGACTTCGGATGGTAGA Chr5:138606370..138606389 60.36 55

*** Putative Vector Insertion (Chr 5: 138606149 - 138606279) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000535291 Chr5:138605817..138606148 TGAAGCCACGAGATATGCAG Chr5:138606017..138606036 59.97 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGTGAAAAGGGACAGACAGC Chr5:138606281..138606301 59.7 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GGTGAAAAGGGACAGACAGC Chr5:138606281..138606301 59.7 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000029730