Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI17897
Trapped Gene
Ard1 (ENSMUSG00000031388)
Vector Insertion
Chr X: 71166710 - 71167094
Public Clones not available
Private Clones OST434103 (lexicon) OST394584 (lexicon) OST50999 (lexicon)
Severity of mutation (?) Insertion after 3% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000699153 (ChrX:71167095..71167167 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GATGAACATCCGCAATGCTA ChrX:71167097..71167116 59.65 45 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000699153 (ChrX:71167095..71167167 -)
Downstram Exon
ENSMUSE00000270106 (ChrX:71166611..71166709 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GATGAACATCCGCAATGCTA ChrX:71167097..71167116 59.65 45 GTGCTGCATGTTCATCAGGT ChrX:71166661..71166680 59.71 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000485553 ChrX:71167095..71167184 GATGAACATCCGCAATGCTA ChrX:71167097..71167116 59.65 45
upstream ENSMUSE00000699153 ChrX:71167095..71167167 GATGAACATCCGCAATGCTA ChrX:71167097..71167116 59.65 45
upstream ENSMUSE00000699155 ChrX:71167095..71167227 GATGAACATCCGCAATGCTA ChrX:71167097..71167116 59.65 45
upstream ENSMUSE00000699159 ChrX:71167095..71167171 GATGAACATCCGCAATGCTA ChrX:71167097..71167116 59.65 45

*** Putative Vector Insertion (Chr X: 71166710 - 71167094) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000270106 ChrX:71166611..71166709 GTGCTGCATGTTCATCAGGT ChrX:71166661..71166680 59.71 50
downstream ENSMUSE00000209030 ChrX:71166322..71166380 CCAAGACGTAGCCCACAATC ChrX:71166307..71166326 60.52 55
downstream ENSMUSE00000209025 ChrX:71165302..71165347 CACATCGTCTGGGTCCTCTT ChrX:71165304..71165323 60.11 55
downstream ENSMUSE00000209029 ChrX:71165093..71165208 ATGCAGGGAGACGTATTTGG ChrX:71165082..71165101 59.96 50
downstream ENSMUSE00000209031 ChrX:71164841..71164885 TGAAAGTTGAGGGTGTTGGA ChrX:71164819..71164838 59.11 45
downstream ENSMUSE00000699158 ChrX:71164841..71164879 TGAAAGTTGAGGGTGTTGGA ChrX:71164819..71164838 59.11 45
downstream ENSMUSE00000464900 ChrX:71163182..71163266 CATACGCATCTTCCCCATCT ChrX:71163195..71163214 59.92 50
downstream ENSMUSE00000622462 ChrX:71162813..71163266 GCCCCTGACCCTAGTAGTCC ChrX:71162843..71162862 59.96 65
downstream ENSMUSE00000647175 ChrX:71162298..71162534 AAGCACGTTGCCTTTGTTCT ChrX:71162423..71162442 59.92 45
downstream ENSMUSE00000332014 ChrX:71162259..71162534 AAGCACGTTGCCTTTGTTCT ChrX:71162423..71162442 59.92 45
downstream ENSMUSE00000699157 ChrX:71162229..71162625 AAGCACGTTGCCTTTGTTCT ChrX:71162423..71162442 59.92 45
downstream ENSMUSE00000699154 ChrX:71162226..71162621 AAGCACGTTGCCTTTGTTCT ChrX:71162423..71162442 59.92 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CCTTACGGGTAATCGCCTTG ChrX:71167032..71167052 61.69 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CAATGCTAGGGTGAGGTGAC ChrX:71167083..71167103 58.16 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000031388