Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI18011
Trapped Gene
2900010J23Rik (ENSMUSG00000044627)
Vector Insertion
Chr 2: 32134336 - 32134652
Public Clones not available
Private Clones OST432161 (lexicon) OST349136 (lexicon) OST250120 (lexicon) OST235433 (lexicon)
OST165415 (lexicon) OST79370 (lexicon) OST33235 (lexicon)
Severity of mutation (?) Insertion after 77% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000695426 (Chr2:32134337..32134651 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
ATCTACAGCCCACAGGGACA Chr2:32134547..32134566 60.53 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000695426 (Chr2:32134337..32134651 -)
Downstram Exon
ENSMUSE00000447332 (Chr2:32134336..32134651 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
ATCTACAGCCCACAGGGACA Chr2:32134547..32134566 60.53 55 TCCAAGAAGCTGCTCAGTCA Chr2:32134552..32134571 59.85 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000695437 Chr2:32143551..32143582 No primer for this exon
upstream ENSMUSE00000447349 Chr2:32143406..32143556 GTCTCCGTCGTCGGTTTCTA Chr2:32143498..32143517 60.26 55
upstream ENSMUSE00000695428 Chr2:32143406..32143533 GTCTCCGTCGTCGGTTTCTA Chr2:32143498..32143517 60.26 55
upstream ENSMUSE00000695434 Chr2:32143117..32143165 GACTTTACCATGGCGGCTAC Chr2:32143126..32143145 59.6 55
upstream ENSMUSE00000712502 Chr2:32143117..32143165 GACTTTACCATGGCGGCTAC Chr2:32143126..32143145 59.6 55
upstream ENSMUSE00000712656 Chr2:32143117..32143165 GACTTTACCATGGCGGCTAC Chr2:32143126..32143145 59.6 55
upstream ENSMUSE00000695421 Chr2:32138209..32138384 CAGCTCTCAAGCTCCAACAA Chr2:32138275..32138294 59.31 50
upstream ENSMUSE00000345216 Chr2:32137206..32137330 TGACATGCTGGACAAGGAGA Chr2:32137225..32137244 60.4 50
upstream ENSMUSE00000695432 Chr2:32137206..32137330 TGACATGCTGGACAAGGAGA Chr2:32137225..32137244 60.4 50
upstream ENSMUSE00000363891 Chr2:32136229..32136323 GCTACCGTGTGATTGAGCTG Chr2:32136302..32136321 59.47 55
upstream ENSMUSE00000695426 Chr2:32134337..32134651 ATCTACAGCCCACAGGGACA Chr2:32134547..32134566 60.53 55
upstream ENSMUSE00000447332 Chr2:32134336..32134651 TGACTGAGCAGCTTCTTGGA Chr2:32134574..32134593 59.85 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CTGTGACTCGAGGCGTTACC Chr2:32134630..32134650 60.85 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CTGTGACTCGAGGCGTTACC Chr2:32134630..32134650 60.85 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000044627