Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI18104
Trapped Gene
Sec14l1 (ENSMUSG00000020823)
Vector Insertion
Chr 11: 116978473 - 116978567
Public Clones not available
Private Clones OST430170 (lexicon) OST391009 (lexicon) OST323853 (lexicon) OST283451 (lexicon)
OST258052 (lexicon) OST198626 (lexicon) OST194128 (lexicon) OST170534 (lexicon)
OST143638 (lexicon) OST134153 (lexicon) OST121434 (lexicon) OST121411 (lexicon)
OST121397 (lexicon) OST121363 (lexicon) OST112854 (lexicon) OST112846 (lexicon)
OST107048 (lexicon) OST49942 (lexicon) OST39030 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000430293 (Chr11:116978474..116978566 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000430293 (Chr11:116978474..116978566 +)
Downstram Exon
ENSMUSE00000707550 (Chr11:116978474..116978566 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000645924 Chr11:116976552..116976665 No primer for this exon

*** Putative Vector Insertion (Chr 11: 116978473 - 116978567) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000430293 Chr11:116978474..116978566 No primer for this exon
downstream ENSMUSE00000707550 Chr11:116978474..116978566 No primer for this exon
downstream ENSMUSE00000109612 Chr11:117002732..117002881 No primer for this exon
downstream ENSMUSE00000109632 Chr11:117002976..117003107 No primer for this exon
downstream ENSMUSE00000109635 Chr11:117004601..117004729 No primer for this exon
downstream ENSMUSE00000669592 Chr11:117005095..117005329 No primer for this exon
downstream ENSMUSE00000645939 Chr11:117006163..117006272 No primer for this exon
downstream ENSMUSE00000645937 Chr11:117008422..117008611 No primer for this exon
downstream ENSMUSE00000645936 Chr11:117009849..117009937 No primer for this exon
downstream ENSMUSE00000645935 Chr11:117010460..117010530 No primer for this exon
downstream ENSMUSE00000645934 Chr11:117011473..117011644 No primer for this exon
downstream ENSMUSE00000645933 Chr11:117011918..117012052 No primer for this exon
downstream ENSMUSE00000645931 Chr11:117014458..117014592 No primer for this exon
downstream ENSMUSE00000645929 Chr11:117016534..117016785 No primer for this exon
downstream ENSMUSE00000645928 Chr11:117017675..117017853 No primer for this exon
downstream ENSMUSE00000661061 Chr11:117018115..117018216 No primer for this exon
downstream ENSMUSE00000712516 Chr11:117018115..117020582 No primer for this exon
downstream ENSMUSE00000645926 Chr11:117018573..117018873 No primer for this exon
downstream ENSMUSE00000645919 Chr11:117020068..117020083 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CCATGGTGCAAAAATACCAG Chr11:116978503..116978523 58.89 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CCATGGTGCAAAAATACCAG Chr11:116978503..116978523 58.89 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000020823