Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI18219
Trapped Gene
AC154532.3-202 (ENSMUSG00000056234)
Vector Insertion
Chr 14: 32990560 - 32991008
Public Clones not available
Private Clones OST427309 (lexicon)
Severity of mutation (?) Insertion after 100% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000484533 (Chr14:32990419..32990559 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
ACTTTCGCCCCGACTGTTAC Chr14:32990456..32990475 61.43 55 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000484533 (Chr14:32990419..32990559 +)
Downstram Exon
ENSMUSE00000690091 (Chr14:32991009..32992438 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
ACTTTCGCCCCGACTGTTAC Chr14:32990456..32990475 61.43 55 GTGTACCGGGATGTGGTTTC Chr14:32991287..32991306 60.1 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000690094 Chr14:32973169..32973196 No primer for this exon
upstream ENSMUSE00000343400 Chr14:32983934..32984088 GGAGTACTTCGGGCTGAACA Chr14:32984044..32984063 60.26 55
upstream ENSMUSE00000121903 Chr14:32985489..32985629 ATAAGTCGCCACCTGGAATG Chr14:32985513..32985532 59.96 50
upstream ENSMUSE00000121900 Chr14:32985793..32985881 No primer for this exon
upstream ENSMUSE00000121906 Chr14:32986075..32986183 GCCAGACCATCACCACATTT Chr14:32986146..32986165 60.79 50
upstream ENSMUSE00000121904 Chr14:32986587..32986676 CACGCGAGCTCCTCAAGTAT Chr14:32986611..32986630 60.56 55
upstream ENSMUSE00000486461 Chr14:32987576..32987719 CAGGACTGGCTTATCCCAAA Chr14:32987678..32987697 60.07 50
upstream ENSMUSE00000121901 Chr14:32989125..32990141 GCTGATTCCTTCCACGTCAT Chr14:32989893..32989912 60.08 50
upstream ENSMUSE00000484533 Chr14:32990419..32990559 ACTTTCGCCCCGACTGTTAC Chr14:32990456..32990475 61.43 55

*** Putative Vector Insertion (Chr 14: 32990560 - 32991008) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000690091 Chr14:32991009..32992438 GTGTACCGGGATGTGGTTTC Chr14:32991287..32991306 60.1 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TTCTAATCGCCTTGCAGCAC Chr14:32990608..32990628 61.44 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GCTCATAAATGTTTTCCGTGAC Chr14:32990595..32990617 58.63 40.91 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000056234