Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI18273
Trapped Gene
Fkbp11 (ENSMUSG00000003355)
Vector Insertion
Chr 15: 98555034 - 98556914
Public Clones CMHD-GT_514G4-3 (cmhd)
Private Clones OST426625 (lexicon) OST426613 (lexicon) OST426609 (lexicon) OST195182 (lexicon)
OST17000 (lexicon) OST2370 (lexicon)
Severity of mutation (?) Insertion after 65% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000132537 (Chr15:98556915..98556985 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000132537 (Chr15:98556915..98556985 -)
Downstram Exon
ENSMUSE00000401953 (Chr15:98554801..98555033 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000132535 Chr15:98558388..98558629 No primer for this exon
upstream ENSMUSE00000132539 Chr15:98558114..98558179 No primer for this exon
upstream ENSMUSE00000132540 Chr15:98557642..98557729 No primer for this exon
upstream ENSMUSE00000132536 Chr15:98557331..98557364 No primer for this exon
upstream ENSMUSE00000132537 Chr15:98556915..98556985 No primer for this exon

*** Putative Vector Insertion (Chr 15: 98555034 - 98556914) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000401953 Chr15:98554801..98555033 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CCAAGAACCTAATCGCCTTG Chr15:98556852..98556872 59.7 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CCTTCTCACCTGGCTTATGG Chr15:98556942..98556962 59.69 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000003355