Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI18330
Trapped Gene
Immp1l (ENSMUSG00000042670)
Vector Insertion
Chr 2: 105748587 - 105770875
Public Clones (sanger)
Private Clones OST425485 (lexicon) OST409544 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000413144 (Chr2:105748481..105748586 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GCTTTGAGACCTCCGTCAGA Chr2:105748510..105748529 60.53 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000413144 (Chr2:105748481..105748586 +)
Downstram Exon
ENSMUSE00000224199 (Chr2:105770876..105771009 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GCTTTGAGACCTCCGTCAGA Chr2:105748510..105748529 60.53 55 CCAGAACACCACGAAGCATA Chr2:105770926..105770945 59.72 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000340821 Chr2:105744836..105744887 CCGAGGAACTTCTGTTGGTG Chr2:105744855..105744874 60.68 55
upstream ENSMUSE00000413144 Chr2:105748481..105748586 GCTTTGAGACCTCCGTCAGA Chr2:105748510..105748529 60.53 55

*** Putative Vector Insertion (Chr 2: 105748587 - 105770875) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000224199 Chr2:105770876..105771009 CCAGAACACCACGAAGCATA Chr2:105770926..105770945 59.72 50
downstream ENSMUSE00000224191 Chr2:105773422..105773510 GGTTCCATTGATGGTCCAGA Chr2:105773447..105773466 60.72 50
downstream ENSMUSE00000224185 Chr2:105777149..105777275 TTTTGGATCACTTGGGCTTT Chr2:105777191..105777210 59.55 40
downstream ENSMUSE00000224176 Chr2:105804337..105804447 No primer for this exon
downstream ENSMUSE00000344184 Chr2:105805421..105805574 GTCACGCAGAAATCCGAAGT Chr2:105805459..105805478 60.26 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TTGTTCAGGATAGTGTTTGCTTG Chr2:105751575..105751598 59.32 39.13 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TTGTTCAGGATAGTGTTTGCTTG Chr2:105751575..105751598 59.32 39.13 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000042670