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About
UniTrap UNI18416
Trapped Gene
Fnbp1 (
ENSMUSG00000075415
)
Vector Insertion
Chr 2: 30881726 - 30884404
Public Clones
not available
Private Clones
OST423866 (lexicon)
Severity of mutation
(?)
Insertion after 100% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000696146 (Chr2:30881727..30884403 -)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
TTCATCGGATCCTGTTCTCC
Chr2:30883479..30883498
60.01
50
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000696146 (Chr2:30881727..30884403 -)
Downstram Exon
ENSMUSE00000696149 (Chr2:30881726..30884403 -)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
TTCATCGGATCCTGTTCTCC
Chr2:30883479..30883498
60.01
50
GGAGAACAGGATCCGATGAA
Chr2:30883457..30883476
60.01
50
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000479781
Chr2:30997299..30997526
CTTTCCGCATTCACAAGGTT
Chr2:30997383..30997402
60.11
45
upstream
ENSMUSE00000662797
Chr2:30997299..30997528
CTTTCCGCATTCACAAGGTT
Chr2:30997383..30997402
60.11
45
upstream
ENSMUSE00000696139
Chr2:30997299..30997322
No primer for this exon
upstream
ENSMUSE00000696122
Chr2:30971745..30971807
ATGGAAGATGGCCTGATGAA
Chr2:30971788..30971807
60.43
45
upstream
ENSMUSE00000696128
Chr2:30965016..30965028
No primer for this exon
upstream
ENSMUSE00000696143
Chr2:30965016..30965029
No primer for this exon
upstream
ENSMUSE00000696142
Chr2:30961422..30961429
No primer for this exon
upstream
ENSMUSE00000281967
Chr2:30960762..30960877
CAAGGAGAGGACGGAGATTG
Chr2:30960787..30960806
59.8
55
upstream
ENSMUSE00000163540
Chr2:30952614..30952670
AACTCGAAGGAAGAGGAGGA
Chr2:30952620..30952639
58.05
50
upstream
ENSMUSE00000163542
Chr2:30951547..30951694
CAGCACGAGGTCATCTCTGA
Chr2:30951617..30951636
60.14
55
upstream
ENSMUSE00000163538
Chr2:30939543..30939605
GGCTCAGCAGCACATAGAAA
Chr2:30939566..30939585
59.18
50
upstream
ENSMUSE00000163541
Chr2:30938494..30938598
GTTTGAGCGGGACTGTAAGG
Chr2:30938568..30938587
59.73
55
upstream
ENSMUSE00000717061
Chr2:30914409..30914537
TACCCACATCCCCAACATCT
Chr2:30914414..30914433
60.05
50
upstream
ENSMUSE00000722142
Chr2:30914409..30914537
TACCCACATCCCCAACATCT
Chr2:30914414..30914433
60.05
50
upstream
ENSMUSE00000696123
Chr2:30914128..30914133
No primer for this exon
upstream
ENSMUSE00000281923
Chr2:30910948..30911094
TGAAGACGTACGCAGAGGTG
Chr2:30911026..30911045
60.05
55
upstream
ENSMUSE00000696119
Chr2:30910948..30911094
TGAAGACGTACGCAGAGGTG
Chr2:30911026..30911045
60.05
55
upstream
ENSMUSE00000568127
Chr2:30909544..30909738
CCAATGAAACGCACAGTGTC
Chr2:30909641..30909660
60.16
50
upstream
ENSMUSE00000696126
Chr2:30909511..30909738
CCAATGAAACGCACAGTGTC
Chr2:30909641..30909660
60.16
50
upstream
ENSMUSE00000662796
Chr2:30908755..30909738
CTCCCAGCTGGTCGTAGAAG
Chr2:30909717..30909736
60.01
60
upstream
ENSMUSE00000506828
Chr2:30908505..30908600
CAAACCCAAAATCCACTGCT
Chr2:30908525..30908544
59.97
45
upstream
ENSMUSE00000568126
Chr2:30908505..30908687
CAAACCCAAAATCCACTGCT
Chr2:30908525..30908544
59.97
45
upstream
ENSMUSE00000696157
Chr2:30908505..30908657
CAAACCCAAAATCCACTGCT
Chr2:30908525..30908544
59.97
45
upstream
ENSMUSE00000696148
Chr2:30900402..30900416
No primer for this exon
upstream
ENSMUSE00000604325
Chr2:30895925..30896034
CAGAAGGAGACGGACCAGAG
Chr2:30895925..30895944
59.98
60
upstream
ENSMUSE00000604324
Chr2:30892676..30892808
AGAAGCTCACCGAAGTCACC
Chr2:30892718..30892737
59.46
55
upstream
ENSMUSE00000604323
Chr2:30892062..30892183
CAGACACACCAGACGGTCAC
Chr2:30892086..30892105
60.21
60
upstream
ENSMUSE00000604322
Chr2:30891638..30891774
CGGATTTCGACGATGAATTT
Chr2:30891696..30891715
59.9
40
upstream
ENSMUSE00000568116
Chr2:30888550..30888708
TTACGTCCCCACTTCCTACG
Chr2:30888580..30888599
59.99
55
upstream
ENSMUSE00000696131
Chr2:30888530..30888708
TTACGTCCCCACTTCCTACG
Chr2:30888580..30888599
59.99
55
upstream
ENSMUSE00000335812
Chr2:30888523..30888708
TTACGTCCCCACTTCCTACG
Chr2:30888580..30888599
59.99
55
upstream
ENSMUSE00000696134
Chr2:30888519..30888708
TTACGTCCCCACTTCCTACG
Chr2:30888580..30888599
59.99
55
upstream
ENSMUSE00000696146
Chr2:30881727..30884403
TTCATCGGATCCTGTTCTCC
Chr2:30883479..30883498
60.01
50
upstream
ENSMUSE00000696138
Chr2:30881726..30888708
AGGCACCATTTCCGTAGTTG
Chr2:30888679..30888698
59.99
50
upstream
ENSMUSE00000696149
Chr2:30881726..30884403
TTCATCGGATCCTGTTCTCC
Chr2:30883479..30883498
60.01
50
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
AGGTTCCTAAAGGCGTGGTG
Chr2:30884384..30884404
61.41
55
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
AGGTTCCTAAAGGCGTGGTG
Chr2:30884384..30884404
61.41
55
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000075415