Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI18419
Trapped Gene
4933406E20Rik (ENSMUSG00000006675)
Vector Insertion
Chr 9: 108483236 - 108484172
Public Clones not available
Private Clones OST423736 (lexicon) OST337889 (lexicon) OST314028 (lexicon)
Severity of mutation (?) Insertion after 59% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000583240 (Chr9:108484173..108484335 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000583240 (Chr9:108484173..108484335 -)
Downstram Exon
ENSMUSE00000583239 (Chr9:108483050..108483235 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000221368 Chr9:108499539..108499931 No primer for this exon
upstream ENSMUSE00000240493 Chr9:108499230..108499311 No primer for this exon
upstream ENSMUSE00000583242 Chr9:108485973..108486163 No primer for this exon
upstream ENSMUSE00000583241 Chr9:108485178..108485274 No primer for this exon
upstream ENSMUSE00000583240 Chr9:108484173..108484335 No primer for this exon

*** Putative Vector Insertion (Chr 9: 108483236 - 108484172) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000583239 Chr9:108483050..108483235 No primer for this exon
downstream ENSMUSE00000583238 Chr9:108482347..108482437 No primer for this exon
downstream ENSMUSE00000583237 Chr9:108482047..108482170 No primer for this exon
downstream ENSMUSE00000583236 Chr9:108481157..108481639 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CTTTAATCGCCTTGCAGCAC Chr9:108484104..108484124 60.9 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GGTGGCCACACAAGTTCTTC Chr9:108484120..108484140 60.56 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000006675