Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI18591
Trapped Gene
Aebp2 (ENSMUSG00000030232)
Vector Insertion
Chr 6: 140582209 - 140582418
Public Clones not available
Private Clones OST420067 (lexicon) OST329967 (lexicon) OST307194 (lexicon) OST283910 (lexicon)
OST274269 (lexicon) OST264060 (lexicon) OST258615 (lexicon) OST252000 (lexicon)
OST240140 (lexicon) OST128040 (lexicon) OST100436 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000608579 (Chr6:140582210..140582417 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CATTCGCTCCATACATGTCG Chr6:140582381..140582400 60.1 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000608579 (Chr6:140582210..140582417 +)
Downstram Exon
ENSMUSE00000608582 (Chr6:140582210..140582417 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CATTCGCTCCATACATGTCG Chr6:140582381..140582400 60.1 50 GAGCGTCCACTGGAAATTGT Chr6:140582263..140582282 60.12 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000608583 Chr6:140571184..140571579 CAGCCTGCCTACAAGTGTCA Chr6:140571449..140571468 60.05 55
upstream ENSMUSE00000714143 Chr6:140572210..140572865 AGGAGGAGGATGACGAGGAG Chr6:140572334..140572353 60.75 60
upstream ENSMUSE00000716728 Chr6:140572210..140572865 AGGAGGAGGATGACGAGGAG Chr6:140572334..140572353 60.75 60
upstream ENSMUSE00000706715 Chr6:140572819..140572865 No primer for this exon

*** Putative Vector Insertion (Chr 6: 140582209 - 140582418) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000608579 Chr6:140582210..140582417 GAGCGTCCACTGGAAATTGT Chr6:140582263..140582282 60.12 50
downstream ENSMUSE00000608582 Chr6:140582210..140582417 GAGCGTCCACTGGAAATTGT Chr6:140582263..140582282 60.12 50
downstream ENSMUSE00000196633 Chr6:140586181..140586288 CGGGGTGTTATACACCTTGC Chr6:140586228..140586247 60.25 55
downstream ENSMUSE00000196631 Chr6:140590693..140590879 TTTCCTCCGCTTGTTCATTC Chr6:140590854..140590873 60.19 45
downstream ENSMUSE00000196630 Chr6:140592469..140592593 AAGCAGATGGCTCGATGTCT Chr6:140592535..140592554 59.98 50
downstream ENSMUSE00000196629 Chr6:140595331..140595398 GGCATCCAATGAAGCAAAAG Chr6:140595392..140595411 60.58 45
downstream ENSMUSE00000196632 Chr6:140596493..140596606 TCCAACAGCAAGGCAGTATCT Chr6:140596593..140596613 59.89 47.62
downstream ENSMUSE00000430513 Chr6:140596493..140596559 No primer for this exon
downstream ENSMUSE00000430510 Chr6:140596561..140596606 AAATGTTTGGGTCCAACAGC Chr6:140596605..140596624 59.84 45
downstream ENSMUSE00000430516 Chr6:140599277..140604002 GGTACCGCTCCCAACAGTAA Chr6:140600710..140600729 59.99 55
downstream ENSMUSE00000546755 Chr6:140599277..140599299 No primer for this exon
downstream ENSMUSE00000608581 Chr6:140599277..140599303 No primer for this exon
downstream ENSMUSE00000689236 Chr6:140625912..140625939 TCACCTTGGGAAATTGAGGA Chr6:140625942..140625961 60.43 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CAATTTCCAGTGGACGCTAA Chr6:140582243..140582263 58.77 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CATAGACAGCACAATTTCCAGTG Chr6:140582232..140582255 59.69 43.48 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000030232