Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI18626
Trapped Gene
Mobp (ENSMUSG00000032517)
Vector Insertion
Chr 9: 120058916 - 120063804
Public Clones not available
Private Clones OST419246 (lexicon) OST247923 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000582965 (Chr9:120058860..120058915 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TCCACAGGAACCTTTCACAA Chr9:120058880..120058899 59.11 45 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000582965 (Chr9:120058860..120058915 +)
Downstram Exon
ENSMUSE00000220404 (Chr9:120063805..120063883 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TCCACAGGAACCTTTCACAA Chr9:120058880..120058899 59.11 45 GTCTCCGCTTGTCTTGCTCT Chr9:120063846..120063865 59.75 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000582965 Chr9:120058860..120058915 TCCACAGGAACCTTTCACAA Chr9:120058880..120058899 59.11 45

*** Putative Vector Insertion (Chr 9: 120058916 - 120063804) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000220404 Chr9:120063805..120063883 GTCTCCGCTTGTCTTGCTCT Chr9:120063846..120063865 59.75 55
downstream ENSMUSE00000349501 Chr9:120076919..120077128 TGCTCGGAGAACTTCTGGTT Chr9:120076987..120077006 59.99 50
downstream ENSMUSE00000633611 Chr9:120077341..120078494 GCCAGCTTGTCTCTTTTTGG Chr9:120078163..120078182 59.99 50
downstream ENSMUSE00000688558 Chr9:120080636..120080651 No primer for this exon
downstream ENSMUSE00000582961 Chr9:120082269..120085209 TGAAACCAAAAGACCCGTTC Chr9:120082590..120082609 59.95 45
downstream ENSMUSE00000633612 Chr9:120089149..120089161 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGTTCACCCTGTACCTCACG Chr9:120058938..120058958 60.42 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TCGCAGGTAAGAGGATTCCA Chr9:120058911..120058931 60.73 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000032517