Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI18710
Trapped Gene
Agrp (ENSMUSG00000005705)
Vector Insertion
Chr 8: 108091627 - 108092115
Public Clones not available
Private Clones OST416638 (lexicon) OST403829 (lexicon) OST388347 (lexicon) OST388330 (lexicon)
OST352291 (lexicon) OST272124 (lexicon) OST264279 (lexicon) OST241884 (lexicon)
OST171676 (lexicon) OST88852 (lexicon) OST57565 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000580400 (Chr8:108092116..108092198 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000580400 (Chr8:108092116..108092198 -)
Downstram Exon
ENSMUSE00000580399 (Chr8:108091506..108091626 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000580400 Chr8:108092116..108092198 No primer for this exon

*** Putative Vector Insertion (Chr 8: 108091627 - 108092115) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000580399 Chr8:108091506..108091626 No primer for this exon
downstream ENSMUSE00000341888 Chr8:108091260..108091392 No primer for this exon
downstream ENSMUSE00000214388 Chr8:108091020..108091102 No primer for this exon
downstream ENSMUSE00000214390 Chr8:108090600..108090838 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CCTTGGTTTCCAGGAACCTT Chr8:108092116..108092136 60.33 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CCTTGGTTTCCAGGAACCTT Chr8:108092116..108092136 60.33 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000005705