Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI18737
Trapped Gene
Mea1 (ENSMUSG00000002768)
Vector Insertion
Chr 17: 46818112 - 46818565
Public Clones not available
Private Clones OST415502 (lexicon) OST377271 (lexicon) OST235596 (lexicon) OST131931 (lexicon)
OST68699 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000607108 (Chr17:46818073..46818111 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000607108 (Chr17:46818073..46818111 +)
Downstram Exon
ENSMUSE00000136758 (Chr17:46818566..46818846 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000607108 Chr17:46818073..46818111 No primer for this exon

*** Putative Vector Insertion (Chr 17: 46818112 - 46818565) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000136758 Chr17:46818566..46818846 No primer for this exon
downstream ENSMUSE00000136760 Chr17:46818958..46819060 No primer for this exon
downstream ENSMUSE00000424791 Chr17:46819668..46820049 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
  No PCR available

Come back to gene ENSMUSG00000002768