Tools for the biologist enabling optimized use of gene trap clones
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About
UniTrap UNI18737
Trapped Gene
Mea1 (
ENSMUSG00000002768
)
Vector Insertion
Chr 17: 46818112 - 46818565
Public Clones
not available
Private Clones
OST415502 (lexicon)
OST377271 (lexicon)
OST235596 (lexicon)
OST131931 (lexicon)
OST68699 (lexicon)
Severity of mutation
(?)
Insertion after 0% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000607108 (Chr17:46818073..46818111 +)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
No primer for this exon
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000607108 (Chr17:46818073..46818111 +)
Downstram Exon
ENSMUSE00000136758 (Chr17:46818566..46818846 +)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
No primer for this exon
No primer for this exon
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000607108
Chr17:46818073..46818111
No primer for this exon
*** Putative Vector Insertion (Chr 17: 46818112 - 46818565) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000136758
Chr17:46818566..46818846
No primer for this exon
downstream
ENSMUSE00000136760
Chr17:46818958..46819060
No primer for this exon
downstream
ENSMUSE00000424791
Chr17:46819668..46820049
No primer for this exon
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
No PCR available
Come back to gene
ENSMUSG00000002768
