Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI18847
Trapped Gene
Comtd1 (ENSMUSG00000021773)
Vector Insertion
Chr 14: 22667538 - 22667762
Public Clones not available
Private Clones OST413096 (lexicon) OST193749 (lexicon)
Severity of mutation (?) Insertion after 28% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000479091 (Chr14:22667763..22667890 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
ACCTGAGGACAATCCCCTGT Chr14:22667822..22667841 60.77 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000479091 (Chr14:22667763..22667890 -)
Downstram Exon
ENSMUSE00000230122 (Chr14:22667432..22667537 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
ACCTGAGGACAATCCCCTGT Chr14:22667822..22667841 60.77 55 TGTTCACAGGTCATCATGGAA Chr14:22667469..22667489 59.95 42.86

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000423812 Chr14:22668001..22668129 TCGCTACTGGTCTCTTGCTG Chr14:22668002..22668021 59.34 55
upstream ENSMUSE00000479091 Chr14:22667763..22667890 ACCTGAGGACAATCCCCTGT Chr14:22667822..22667841 60.77 55

*** Putative Vector Insertion (Chr 14: 22667538 - 22667762) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000230122 Chr14:22667432..22667537 TGTTCACAGGTCATCATGGAA Chr14:22667469..22667489 59.95 42.86
downstream ENSMUSE00000230116 Chr14:22667224..22667342 CCGAGTAGCCCGTGAAAGTA Chr14:22667300..22667319 60.26 55
downstream ENSMUSE00000120749 Chr14:22667095..22667149 AAGGTCGATCTTCTGCTCCA Chr14:22667101..22667120 59.95 50
downstream ENSMUSE00000120743 Chr14:22666836..22666969 TGCAGACAGCGCTCGTAGTA Chr14:22666851..22666870 60.91 55
downstream ENSMUSE00000345498 Chr14:22666403..22666649 GTTCCGCACACATTCAACAG Chr14:22666562..22666581 60.16 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TAATCGCCTTGCAGCACATC Chr14:22667691..22667711 62.25 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector

Come back to gene ENSMUSG00000021773